A pneumonia outbreak associated with a new coronavirus of probable bat origin
A pneumonia outbreak associated with a new coronavirus of probable bat origin
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与可能源自蝙蝠的新型冠状病毒相关的肺炎爆发
DOI：
10.1038/s41586-020-2012-7
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发表时间：
2020-02-03
期刊：
影响因子：
64.8
通讯作者：
Shi, Zheng-Li
中科院分区：
文献类型：
--
作者：
Zhou, Peng;Yang, Xing-Lou;Shi, Zheng-Li
自18年前严重急性呼吸系统综合征（SARS）爆发以来，在其天然宿主蝙蝠中发现了大量与SARS相关的冠状病毒（sarsr - cov）（1-4）。先前的研究表明，一些蝙蝠sars - cov具有感染人类的潜力（5-7）。在这里，我们报告了一种新型冠状病毒（2019-nCoV）的鉴定和特征，该冠状病毒在中国武汉引起了人类急性呼吸综合征的流行。该流行病始于2019年12月12日，截至2020年1月26日，已造成2794例实验室确诊感染，其中80例死亡。在疫情的早期阶段，从5名患者身上获得了全长基因组序列。序列几乎相同，与SARS-CoV的序列同源性为79.6%。此外，我们发现2019-nCoV在全基因组水平上与蝙蝠冠状病毒相同96%。7个保守的非结构蛋白结构域的成对蛋白序列分析表明该病毒属于sars - cov种属。此外，从1例危重患者支气管肺泡灌洗液中分离到的2019-nCoV病毒可被多例患者血清中和。值得注意的是，我们证实2019-nCoV使用与SARS-CoV相同的细胞进入受体血管紧张素转换酶II （ACE2）。
Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number of SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats(1-4). Previous studies have shown that some bat SARSr-CoVs have the potential to infect humans(5-7). Here we report the identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China. The epidemic, which started on 12 December 2019, had caused 2,794 laboratory-confirmed infections including 80 deaths by 26 January 2020. Full-length genome sequences were obtained from five patients at an early stage of the outbreak. The sequences are almost identical and share 79.6% sequence identity to SARS-CoV. Furthermore, we show that 2019-nCoV is 96% identical at the whole-genome level to a bat coronavirus. Pairwise protein sequence analysis of seven conserved non-structural proteins domains show that this virus belongs to the species of SARSr-CoV. In addition, 2019-nCoV virus isolated from the bronchoalveolar lavage fluid of a critically ill patient could be neutralized by sera from several patients. Notably, we confirmed that 2019-nCoV uses the same cell entry receptor-angiotensin converting enzyme II (ACE2)-as SARS-CoV.