Self-organized optical device driven by motor proteins
Self-organized optical device driven by motor proteins
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DOI：
10.1073/pnas.1306281110
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发表时间：
2013-10-08
影响因子：
11.1
通讯作者：
Hiratsuka, Yuichi
中科院分区：
文献类型：
--
作者：
Aoyama, Susumu;Shimoike, Masahiko;Hiratsuka, Yuichi
蛋白质分子根据它们的组合和排列产生不同的功能，这在活细胞中是显而易见的。因此，它们在未来的设备中具有很大的应用潜力。然而，目前很难构建大量不同蛋白质分子协同工作的系统。作为应对这一挑战的一种方法，我们将蛋白质分子排列在人工微结构中，并组装了一种受鱼类黑色素细胞分子系统启发的光学装置。我们制备了细胞样微室阵列，每个微室的中心都含有微管种子的支架。通过从固定的微管种子聚合微管蛋白，我们在腔室中获得了放射状排列的微管。随后，我们制备了与动力蛋白马达相关的色素颗粒，并将它们附着在径向微管阵列上，这使得黑色素细胞样系统。当ATP被添加到系统中时，由于色素的主动运输，腔室的颜色模式成功地改变。此外，作为该系统的应用，在光学单元的阵列上执行图像形成。这项研究表明，适当设计的微结构有利于分子的排列和自组织，并使功能分子系统的组装成为可能。
Protein molecules produce diverse functions according to their combination and arrangement as is evident in a living cell. Therefore, they have a great potential for application in future devices. However, it is currently very difficult to construct systems in which a large number of different protein molecules work cooperatively. As an approach to this challenge, we arranged protein molecules in artificial microstructures and assembled an optical device inspired by a molecular system of a fish melanophore. We prepared arrays of cell-like microchambers, each of which contained a scaffold of microtubule seeds at the center. By polymerizing tubulin from the fixed microtubule seeds, we obtained radially arranged microtubules in the chambers. We subsequently prepared pigment granules associated with dynein motors and attached them to the radial microtubule arrays, which made a melanophore-like system. When ATP was added to the system, the color patterns of the chamber successfully changed, due to active transportation of pigments. Furthermore, as an application of the system, image formation on the array of the optical units was performed. This study demonstrates that a properly designed microstructure facilitates arrangement and self-organization of molecules and enables assembly of functional molecular systems.