NMR of proteins and nucleic acids
NMR of proteins and nucleic acids
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DOI：
10.1039/9781782622758-00348
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发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
P. Simpson
中科院分区：
文献类型：
--
作者：
P. Simpson
核磁共振（NMR）光谱学可以为生物系统提供令人眼花缭乱的数据财富，这使得它在每年发表的数千篇研究中得到了应用。本综述重点介绍了一些在结构生物学领域使用该技术的关键论文，这些论文在截至2014年5月的12个月期间出现在同行评议的期刊上。本章的第一部分涉及提出方法学进展的论文，特别是在样本生产和新的或改进的数据分析算法方面。随后，总结了一些研究，根据其特定的生物学兴趣或由于所调查系统的挑战性而选择。在空间的限制下，尽可能地描述所使用的方法。因此，该评论旨在为读者提供结构生物学中核磁共振“最新技术”的快照。
The dazzling wealth of data that Nuclear Magnetic Resonance (NMR) spectroscopy can provide on biological systems leads to its application in many thousands of studies published annually. This review highlights a number of key papers which used the technique in the field of Structural Biology, appearing in peer-reviewed journals over the 12 month period up until May 2014. The first part of the chapter deals with papers which present methodological advances, in particular in sample production and new or improved algorithms for data analysis. Subsequently, a number of studies are summarised, selected for their particular biological interest or due to the challenging nature of the system under investigation. As far as possible within the constraints of space, the methods used are described. The review is thus intended to provide the reader with a snapshot of the “state of the art” of NMR in Structural Biology.