DNA barcoding reflects the diversity and variety of brooding traits of fish species in the family Syngnathidae along China’s coast
DNA barcoding reflects the diversity and variety of brooding traits of fish species in the family Syngnathidae along China’s coast
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DOI：
10.1016/j.fishres.2016.09.015
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发表时间：
2017
影响因子：
2.4
通讯作者：
Yanhong Zhang;G. Qin;Huixian Zhang;Xin Wang;Q. Lin
中科院分区：
文献类型：
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作者：
Yanhong Zhang;G. Qin;Huixian Zhang;Xin Wang;Q. Lin
DNA条形码为物种标记和识别提供了快速准确的评估。在这里，我们研究了海龙科样本中DNA条形码的表现，海龙科是一种独特的雄性怀孕鱼类。使用细胞色素c氧化酶亚基I（COI）基因的649个碱基对片段生成总共1002个DNA条形码。所有物种都与不同的DNA条形码相关，可以很容易地区分; COI条形码簇中的7个代表了提交给生命数据系统条形码（BOLD）和GenBank数据库的第一个物种记录。在COI序列的邻接树中，出现了两个主要的类群（Gastrophori和Urophori），这两个类群也可以根据它们的育儿袋进行分类。而尾育雏的蓝斑海马和干育雏的双棘海龙则分别与腹足类和尾足类反聚在一起。海马和尖嘴鱼的序列都显示出高频率的核苷酸替换。海龙的核苷酸替换概率高于所有海马。然而，我们在任何数据集中均未发现基于COI基因的阳性选择信号。我们的研究结果支持DNA条形码作为一种有效的分子工具，实现更好的监测，保护和管理渔业。
DNA barcoding offers a rapid and accurate assessment for species labelling and identification. Here, we investigated the performance of DNA barcodes in a sample of Syngnathidae, a unique group of fish with male pregnancy. A total of 1002 DNA barcodes using 649 base-pair fragments of the cytochrome c oxidase subunit I (COI) gene were generated. All species were associated with distinct DNA barcode and could be readily distinguished; seven of the COI barcode clusters represented the first species records submitted to the Barcode of Life Data Systems (BOLD) and GenBank databases. In the Neighbor-joining tree of COI sequences, two major clusters (Gastrophori and Urophori) were displayed, which could also be classified by their brood pouches. However, the tail-broodingHippichthys cyanospilusand trunk-broodingSyngnathoides biaculeatuswere reverse-clustered together with the Gastrophori and the Urophori, respectively. Both seahorse and pipefish sequences showed high frequencies of nucleotide substitutions. The probability of nucleotide substitutions, those in pipefish were higher than that of all seahorses. However, we identified no signal for positive selection based on the COI gene in any of the data sets. Our results supported DNA barcoding as an efficient molecular tool for achieving better monitoring, conservation, and management of fisheries.