Triacylglycerol profiling of marine microalgae by mass spectrometry
Triacylglycerol profiling of marine microalgae by mass spectrometry
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DOI：
10.1194/jlr.d018408
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发表时间：
2011-11-01
影响因子：
6.5
通讯作者：
Franz, Annaliese K.
中科院分区：
文献类型：
--
作者：
Danielewicz, Megan A.;Anderson, Lisa A.;Franz, Annaliese K.
我们提出了一种测定产油盐水微藻的三酰甘油（TAG）谱的方法，这些微藻与生物燃料、生物活性脂质和高价值脂基化学前体的生产有关。本文描述了一种利用快速、简单固相萃取（SPE）去除叶绿素的技术，并利用MALDI飞行时间（TOF）质谱（MS）、ESI线性离子阱-轨道阱（LTQ Orbitrap）质谱和H-1核磁共振光谱直接比较了四种微藻（三角褐藻、盐绿藻、眼绿藻和水四藻）完整的TAG组成。直接质谱分析对比较三酰甘油的多不饱和脂肪酸（PUFA）组成特别有效，因为氧化通常会在衍生化时降解样品。使用这些方法，我们观察到，相对于其他微藻，T. suecica含有显著的PUFA水平。该方法适用于微藻TAG谱的高通量质谱筛选，可能有助于生物燃料的商业开发。——丹尼尔维奇，m.a.安德森，a.k.弗兰兹海洋微藻的三酰甘油质谱分析。[j] .油脂杂志。2011。52: 2101 - 2108。
We present a method for the determination of triacylglycerol (TAG) profiles of oleaginous saltwater microalgae relevant for the production of biofuels, bioactive lipids, and high-value lipid-based chemical precursors. We describe a technique to remove chlorophyll using quick, simple solid phase extraction (SPE) and directly compare the intact TAG composition of four microalgae species (Phaeodactylum tricornutum, Nannochloropsis salina, Nannochloropsis oculata, and Tetraselmis suecica) using MALDI time-of-flight (TOF) mass spectrometry (MS), ESI linear ion trap-orbitrap (LTQ Orbitrap) MS, and H-1 NMR spectroscopy. Direct MS analysis is particularly effective to compare the polyunsaturated fatty acid (PUFA) composition for triacylglycerols because oxidation can often degrade samples upon derivatization. Using these methods, we observed that T. suecica contains significant PUFA levels with respect to other microalgae. This method is applicable for high-throughput MS screening of microalgae TAG profiles and may aid in the commercial development of biofuels.-Danielewicz, M. A., L. A. Anderson, and A. K. Franz. Triacylglycerol profiling of marine microalgae by mass spectrometry. J. Lipid Res. 2011. 52: 2101-2108.