Phylogenomics provides strong evidence for relationships of butterflies and moths
Phylogenomics provides strong evidence for relationships of butterflies and moths
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DOI：
10.1098/rspb.2014.0970
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发表时间：
2014-08-07
影响因子：
4.7
通讯作者：
Breinholt, Jesse W.
中科院分区：
文献类型：
--
作者：
Kawahara, Akito Y.;Breinholt, Jesse W.
蝴蝶和飞蛾是最受欢迎和最有魅力的昆虫之一。鳞翅目昆虫约有16万种，其中许多是重要的模式生物。以前对鳞翅目进化的研究并没有自信地将蝴蝶放在一起，并且在巨大多样性分支Ditrysia中的许多超科之间的关系仍然很大程度上不确定。我们生成了一个分子数据集与46个分类群，结合33个新的转录组与13个可用的基因组，转录组和表达序列标签（EST）。使用HaMStR与鳞翅目特异性核心直向同源物的单拷贝位点集，我们确定了2696个基因纳入到昆虫基因组分析。所有基因，所有分类单元数据集的核苷酸和氨基酸产生了几乎相同的，良好的支持树。蝴蝶（蝶总科）的单系性得到了有力的支持，这一组包括跳跃者（弄蝶科）和神秘的蝴蝶-蛾（Hedylidae）。蝴蝶被放置在姐妹的其余obtectomoptera鳞翅目，后者被分组大于或等于87%的自助支持。在四个最多样化的大型异角类超家族之间建立可靠的关系以前是具有挑战性的，但我们恢复了以下关系的100%自举支持：（（Geometroidea，Noctuoidea），（Geometroidea，Lasiocampoidea））。我们提出了第一个强大的，基于转录组的鳞翅目的树，强烈矛盾的蝴蝶的历史位置，并提供了一个进化的框架，基因组，发育和生态研究，这种不同的昆虫秩序。
Butterflies and moths constitute some of the most popular and charismatic insects. Lepidoptera include approximately 160 000 described species, many of which are important model organisms. Previous studies on the evolution of Lepidoptera did not confidently place butterflies, and many relationships among superfamilies in the megadiverse clade Ditrysia remain largely uncertain. We generated a molecular dataset with 46 taxa, combining 33 new transcriptomes with 13 available genomes, transcriptomes and expressed sequence tags (ESTs). Using HaMStR with a Lepidoptera-specific core-orthologue set of single copy loci, we identified 2696 genes for inclusion into the phylogenomic analysis. Nucleotides and amino acids of the all-gene, all-taxon dataset yielded nearly identical, well-supported trees. Monophyly of butterflies (Papilionoidea) was strongly supported, and the group included skippers (Hesperiidae) and the enigmatic butterfly-moths (Hedylidae). Butterflies were placed sister to the remaining obtectomeran Lepidoptera, and the latter was grouped with greater than or equal to 87% bootstrap support. Establishing confident relationships among the four most diverse macro-heteroceran superfamilies was previously challenging, but we recovered 100% bootstrap support for the following relationships: ((Geometroidea, Noctuoidea), (Bombycoidea, Lasiocampoidea)). We present the first robust, transcriptome-based tree of Lepidoptera that strongly contradicts historical placement of butterflies, and provide an evolutionary framework for genomic, developmental and ecological studies on this diverse insect order.