Analysis of photoreceptor outer segment phagocytosis by RPE cells in culture.
Analysis of photoreceptor outer segment phagocytosis by RPE cells in culture.
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DOI：
10.1007/978-1-62703-080-9_20
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Finnemann, Silvia C
中科院分区：
文献类型：
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作者：
Mao, Yingyu;Finnemann, Silvia C
视网膜色素上皮细胞（RPE）是自然界中最活跃的吞噬细胞之一。原代RPE和稳定的RPE细胞系提供了与原位RPE具有相同吞噬机制的实验模型系统。在分离的光感受器外段片段（POS）的实验刺激下，这些细胞迅速有效地识别、结合、内化和消化POS。在这里，我们描述了从猪眼睛中分离POS并将POS喂给培养的RPE细胞的实验过程。此外，我们描述了三种不同的互补方法来量化RPE细胞对POS的总摄取，并区分表面结合的POS和吞没的POS。
Retinal pigment epithelial (RPE) cells are among the most actively phagocytic cells in nature. Primary RPE and stable RPE cell lines provide experimental model systems that possess the same phagocytic machinery as RPE in situ. Upon experimental challenge with isolated photoreceptor outer segment fragments (POS), these cells promptly and efficiently recognize, bind, internalize, and digest POS. Here, we describe experimental procedures to isolate POS from porcine eyes and to feed POS to RPE cells in culture. Furthermore, we describe three different and complementary methods to quantify total POS uptake by RPE cells and to discriminate surface-bound from engulfed POS.