Acute resistance exercise increases skeletal muscle angiogenic growth factor expression
Acute resistance exercise increases skeletal muscle angiogenic growth factor expression
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DOI：
10.1111/j.1748-1716.2007.01723.x
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发表时间：
2007-10-01
影响因子：
6.3
通讯作者：
Hickner, R. C.
中科院分区：
文献类型：
--
作者：
Gavin, T. P.;Drew, J. L.;Hickner, R. C.
目的：有氧运动和阻力运动训练都能促进骨骼肌血管生成。急性有氧运动会增加多种促血管生成途径，最明显的是血管内皮生长因子（VEGF）的增加。我们假设急性抗阻运动也会增加骨骼肌血管生成生长因子 [VEGF 和血管生成素 (Ang)] 的表达。方法：对 7 名年轻、久坐的个体在抗阻运动前以及抗阻运动后 0、2 和 4 小时进行股外侧肌活检和抽血，以测量 VEGF； VEGF 受体 [KDR、Flt-1 和神经毡蛋白 1 (Nrp1)]； Ang1和Ang2；以及血管生成素受体 - Tie2 的表达。抗阻运动包括渐进式膝伸肌 (KE) 运动，以确定一次最大重复次数 (1-RM)，然后以 1-RM 的 60-80% 进行三组 10 次重复 (3 x 10) KE 运动。 结果：抗阻运动在 2 小时和 4 小时显着增加骨骼肌 VEGF mRNA 和蛋白质以及血浆 VEGF 蛋白。抗阻运动在 4 小时增加 KDR mRNA 和 Tie2 mRNA，在 2 小时和 4 小时增加 Nrp1 mRNA。骨骼肌 Flt-1、Ang1、Ang2 和 Ang2/Ang1 比率 mRNA 未因抗阻运动而改变。结论：这些研究结果表明，急性抗阻运动会增加骨骼肌 VEGF、VEGF 受体和血管生成素受体的表达。响应急性阻力运动的肌肉血管生成生长因子表达的增加在时间和幅度上与急性有氧运动的响应相似，并且与促进肌肉血管生成的阻力运动一致。
Aims: Both aerobic and resistance exercise training promote skeletal muscle angiogenesis. Acute aerobic exercise increases several pro-angiogenic pathways, the best characterized being increases in vascular endothelial growth factor (VEGF). We hypothesized that acute resistance exercise also increases skeletal muscle angiogenic growth factor [VEGF and angiopoietin (Ang)] expression.Methods: Seven young, sedentary individuals had vastus lateralis muscle biopsies and blood drawn prior to and at 0, 2 and 4 h post-resistance exercise for the measurement of VEGF; VEGF receptor [KDR, Flt-1 and neuropilin 1 (Nrp1)]; Ang1 and Ang2; and the angiopoietin receptor - Tie2 expression. Resistance exercise consisted of progressive knee extensor (KE) exercise to determine one repetition maximum (1-RM) followed by three sets of 10 repetitions (3 x 10) of KE exercise at 60-80% of 1-RM.Results: Resistance exercise significantly increased skeletal muscle VEGF mRNA and protein and plasma VEGF protein at 2 and 4 h. Resistance exercise increased KDR mRNA and Tie2 mRNA at 4 h and Nrp1 mRNA at 2 and 4 h. Skeletal muscle Flt-1, Ang1, Ang2 and Ang2/Ang1 ratio mRNA were not altered by resistance exercise.Conclusions: These findings suggest that acute resistance exercise increases skeletal muscle VEGF, VEGF receptor and angiopoietin receptor expression. The increases in muscle angiogenic growth factor expression in response to acute resistance exercise are similar in timing and magnitude with responses to acute aerobic exercise and are consistent with resistance exercise promoting muscle angiogenesis.