Molecular, histologic, and gross phenotype of skin wound healing in red Duroc pigs reveals an abnormal healing phenotype of hypercontracted, hyperpigmented scarring
Molecular, histologic, and gross phenotype of skin wound healing in red Duroc pigs reveals an abnormal healing phenotype of hypercontracted, hyperpigmented scarring
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DOI：
10.1111/j.1067-1927.2004.012311.x
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发表时间：
2004-05-01
影响因子：
2.9
通讯作者：
Hart, DA
中科院分区：
文献类型：
--
作者：
Gallant, CL;Olson, ME;Hart, DA
在多个水平上表现出独特的愈合表型。大体和组织学分析显示，全层和深层真皮（1.8mm深）伤口均通过形成过度收缩、色素沉着过度的瘢痕而愈合。使用逆转录酶聚合酶链反应和猪特异性引物组的分子分析揭示了I型和III型胶原、热休克蛋白47、骨形态发生蛋白-1、几种蛋白聚糖、和金属蛋白酶1-3的组织抑制剂都显示出独特的双相模式的mRNA表达相比，以前的结果与约克郡猪。该模式的特征在于在创伤后早期表达的初始峰值，随后在第28-42天恢复到接近正常水平，然后在第56-70天mRNA水平的第二次增加。天狼星红染色和偏光显微镜观察到，第二阶段基因表达增加与胶原沉积增加相关。逆转录聚合酶链反应分析也显示了延长基质金属蛋白酶-2的表达相比，以前的研究结果在约克郡菌株。与红色杜洛克表型相关的遗传学和分子生物学的进一步表征可能提供对异常人类伤口愈合的洞察。
exhibit a unique healing phenotype at multiple levels. Gross and histologic analysis revealed that full-thickness and deep dermal (1.8 mm deep) wounds both heal via formation of hypercontracted, hyperpigmented scars, Molecular analysis using reverse-transcriptase polymerase chain reaction and porcine-specific primer sets revealed that types I and III collagen, heat shock protein 47, bone morphogenetic protein-1, several proteoglycans, and tissue inhibitor of metalloproteinases 1-3 all showed a unique biphasic pattern of mRNA expression compared to previous results with Yorkshire pigs. This pattern was characterized by an initial peak of expression early after wounding, followed by a return to near-normal levels by days 28-42, and then a second increase in mRNA levels at days 56-70. The second phase of increased gene expression correlated with an increased collagen deposition as observed by picrosirius red staining and polarizing light microscopy. Reverse-transcriptase polymerase chain reaction analysis also revealed a prolonged expression of matrix metalloproteinase-2 compared to previous findings in the Yorkshire strain. Further characterization of the genetics and molecular biology associated with the red Duroc phenotype may provide insight into aberrant human wound healing.