Molecular neuroimaging of post-injury plasticity.
Molecular neuroimaging of post-injury plasticity.
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伤害后塑性的分子神经影像学。
DOI：
10.1007/s12031-014-0347-y
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Pelled G
中科院分区：
文献类型：
--
作者：
Jouroukhin Y;Nonyane BA;Gilad AA;Pelled G
神经损伤引起初级躯体感觉皮层（S1）神经元活动的长期变化，这通常被认为是感觉功能障碍的起源。然而，这种现象背后的细胞机制仍不清楚。C-fos基因是一个即刻早期基因，在脑可塑性中起重要作用。通过开发一种新的平台来实时成像体内基因表达的变化，我们研究了损伤是否会调节S1第V层中c-fos的水平，因为以前的研究表明这些神经元特别容易受到损伤。黄色荧光蛋白ZsYellow 1在c-fos启动子的调控下在整个大鼠脑内表达。利用能够进行脑深部成像的基于纤维的共聚焦显微镜，同时收集局部场电位。在成年大鼠（n=10）肢体去神经支配后的几周内，与对照组相比，对完整肢体的感觉刺激诱导位于损伤对侧（受影响的，27.6±3个细胞）和同侧（8.6±3个细胞）的S1中的细胞中c-fos基因表达显著增加（n=10，分别为13.4±3和1.0±1，p值<0.05）。因此，我们证明了损伤激活了参与重塑神经元连接的细胞机制，这可能转化为神经康复潜力。
Nerve injury induces long-term changes in neuronal activity in the primary somatosensory cortex (S1), which has often been implicated as the origin of sensory dysfunction. However, the cellular mechanisms underlying this phenomenon remain unclear. C-fos is an immediate early gene, which has been shown to play an instrumental role in plasticity. By developing a new platform to image real-time changes in gene expression in vivo, we investigated whether injury modulates the levels of c-fos in layer V of S1, since previous studies have suggested that these neurons are particularly susceptible to injury. The yellow fluorescent protein, ZsYellow1, under the regulation of the c-fos promoter, was expressed throughout the rat brain. A fiber-based confocal microscope that enabled deep brain imaging was utilized and local field potential were collected simultaneously. In the weeks following limb denervation in adult rats (n=10), sensory stimulation of the intact limb induced significant increases in c-fos gene expression in cells located in S1, both contralateral (affected, 27.6±3 cells) and ipsilateral (8.6±3 cells) to the injury, compared to controls (n=10, 13.4±3 and 1.0±1, respectively, p-value <0.05). Thus, we demonstrated that injury activates cellular mechanisms that are involved in reshaping neuronal connections and this may translate to neurorehabilitative potential.
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影响因子：
4.8
作者：
Fujihara, Hiroaki;Ueta, Yoichi;Murphy, David
通讯作者：
Murphy, David
影响因子：
5.3
作者：
Barth, AL;Gerkin, RC;Dean, KL
通讯作者：
Dean, KL
影响因子：
5.3
作者：
Karl, A;Birbaumer, N;Flor, H
通讯作者：
Flor, H
DOI：
10.1002/neu.480260312
发表时间：
1995-03-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CURRAN, T;MORGAN, JI
通讯作者：
MORGAN, JI
影响因子：
4.2
作者：
Gu, ZZ;Pan, YC;Liu, PK
通讯作者：
Liu, PK