CONSTITUTIVE EXPRESSION OF CD69 IN INTERSPECIES T-CELL HYBRIDS AND LOCUS ASSIGNMENT TO HUMAN CHROMOSOME-12
CONSTITUTIVE EXPRESSION OF CD69 IN INTERSPECIES T-CELL HYBRIDS AND LOCUS ASSIGNMENT TO HUMAN CHROMOSOME-12
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DOI：
10.1007/bf00215288
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发表时间：
1992-06-01
期刊：
影响因子：
3.2
通讯作者：
ACCOLLA, RS
中科院分区：
文献类型：
--
作者：
CAMBIAGGI, C;SCUPOLI, MT;ACCOLLA, RS
在这项研究中，我们描述了人类激活的成熟T细胞与小鼠胸腺瘤细胞BW5147之间的异种体细胞杂交的产生和特征。在杂交种中观察到人类染色体的优先分离。对两个杂交种及其克隆的表型分析表明，在同一细胞中共表达了CD4和CD69抗原。对一个信息丰富的杂交种家族的分离分析以及随后的分子和核型研究清楚地表明，编码CD69抗原的基因定位在人类12号染色体上。尽管CD69抗原的表达是T淋巴细胞激活后的早期事件，并在没有外源刺激的情况下迅速下降，但在本研究描述的杂交种中，CD69抗原的表达是结构性的，与以前在早期胸腺细胞前体细胞和成熟胸腺细胞中发现的类似。在这方面，值得注意的是，杂交细胞在培养中的行为强烈表明，胸腺来源的小鼠肿瘤细胞基因组在控制人CD69的结构性表达方面具有显性影响。因此，这些杂交体可以提供一个系统来研究控制这种激活抗原的表达和功能的遗传和分子机制。
In this study we describe the generation and characterization of interspecies somatic cell hybrids between human activated mature T cells and mouse BW5147 thymoma cells. A preferential segregation of human chromosomes was observed in the hybrids. Phenotypic analysis of two hybrids and their clones demonstrated coexpression of CD4 and CD69 antigens in the same cells. Segregation analysis of an informative family of hybrids followed by molecular and karyotype studies clearly demonstrated that the locus encoding CD69 antigen mapped to human chromosome 12. Although the expression of CD69 antigen is an early event after T-lymphocyte activation and rapidly declines in absence of exogenous stimuli, in the hybrids described in this study the expression was constitutive, similarly to what was previously found in early thymocyte precursors and mature thymocytes. In this respect it was important to note that the behavior of the hybrids in culture strongly suggested a dominant influence of the thymus-derived mouse tumor cell genome in controlling the constitutive expression of human CD69. These hybrids may thus provide a system to study the genetic and molecular mechanisms controlling the expression and function of this activation antigen.