Effect of acute mucosal exposure to Lactobacillus rhamnosus GG on human colonic smooth muscle cells
Effect of acute mucosal exposure to Lactobacillus rhamnosus GG on human colonic smooth muscle cells
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DOI：
10.1097/mcg.0b013e31817e1cac
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发表时间：
2008-09-01
影响因子：
2.9
通讯作者：
Cicala, Michele
中科院分区：
文献类型：
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作者：
Guarino, Michele Pier Luca;Altomare, Annamaria;Cicala, Michele
目的：为了确定人结肠粘膜暴露于美国典型培养物保藏中心（ATCC）53103的鼠李糖乳杆菌GG（LGG）是否可能影响肠肌细胞收缩性。剥离后，将粘膜和粘膜下层在2个腔室之间缩放，粘膜的腔侧朝上并覆盖5 mL Krebs溶液，粘膜下层面朝下并覆盖20 mL Krebs溶液。将LGG或正常下层（N-下层）加入粘膜的腔侧30分钟。将从环形肌层分离的平滑肌细胞（SMC）暴露于培养液中30分钟，培养液来自预先暴露于N-培养液或LGG（36 × 10 - 9菌落形成单位/mL）（LGG-培养液）的粘膜下层室。结果：平滑肌细胞暴露于N-下质后，对乙酰胆碱（Ach）的剂量反应（最大收缩率：1 μ M Ach时为32% ± 5%）与未刺激的平滑肌细胞相似。暴露于LGG-undernatant导致18% +/- 3%的细胞缩短和78% +/- 7%的最大乙酰胆碱诱导的收缩抑制。当SMCs直接暴露于LGG，一个显着的损害收缩（70% +/-5%，与对照细胞相比）和剂量依赖性和时间依赖性缩短observed.Conclusions：急性暴露后的结肠粘膜LGG，SMCs的显着缩短，观察到可能有助于减少收缩反应乙酰胆碱。需要进一步的研究来建立这种作用的机制，这可以解释益生菌在肠道疾病中的临床疗效。
Aim: To define whether human colonic mucosa exposure to Lactobacillus rhamnosus GG (LGG), American Type Culture Collection (ATCC) 53103, may influence intestinal muscle cell contractility.Methods: Human colon specimens were obtained from disease-free margins of resected segments for cancer. The mucosa and submucosa, after dissection, were scaled between 2 chambers, with the luminal side of the mucosa facing upward and covered with 5 mL of Krebs solution and the submucosal side facing downward into 20 mL of Krebs solution. LGG or normal undernatant (N-undernatant) were added to the luminal side of the mucosa for 30 minutes. Smooth muscle cells (SMCs), isolated from the circular muscle layer, were exposed to undernatant for 30 minutes from the submucosal chamber of mucosa that was either preexposed to N-undernatant or to LGG (36 x 10(-9) colony forming units/mL) (LGG-undernatant). Acetylcholine (Ach) dose-response was obtained for SMCs.Results: SMCs exposed to N-undernatant presented a dose-response to Ach (maximal contraction: 32% +/- 5% with 1-mu M Ach) that is similar to unstimulated SMCs. Exposure to LGG-undernatant resulted both in an 18% +/- 3% cell shortening and a 78% +/- 7% inhibition of maximal Ach-induced contraction. When SMCs were directly exposed to LGG, a significant impairment of contraction (70% +/- 5%, compared with control cells) and a dose-dependent and time-dependent shortening were observed.Conclusions: After acute exposure of colonic mucosa to LGG, a significant shortening of SMCs is observed that possibly contributes to the reduced contractile response to Ach. Further studies are needed to establish the mechanisms of this effect that could account for the clinical efficacy of probiotics in intestinal disorders.