Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model
Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model
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使用小鼠模型进行特异性 IgY 治疗脂多糖诱导的内毒素血症的功效
DOI：
10.1111/j.1365-2672.2011.05155.x
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Xu, Y. -P.
中科院分区：
文献类型：
--
作者：
Zhen, Y. -H.;Fang, R.;Xu, Y. -P.
目的：为评价特异性卵黄免疫球蛋白（IgY）治疗脂多糖（LPS）诱导的小鼠内毒素血症的效果，采用甲醛灭活的大肠杆菌O 111免疫鸡卵黄，获得特异性IgY，ELISA法检测其与LPS的结合活性。通过腹腔注射LPS诱导小鼠内毒素血症，剂量为20 mg kg（-1）用于测量存活率，10 mg kg（-1）用于测量细胞因子。用200 mg/kg特异性IgY或5 mg/kg地塞米松处理的小鼠的存活率为70%，而生理盐水处理组的小鼠无一存活超过7天。特异性IgY能显著降低内毒素血症小鼠血清中肿瘤坏死因子-α（TNF-α）水平（P <0.05），升高白细胞介素-10（IL-10）水平（P <0.05）。特异性IgY对TNF-α的影响小于地塞米松，而其增加IL-10的作用强于地塞米松。结论：特异性IgY可提高内毒素血症小鼠的存活率，下调血清中TNF-α的表达，上调血清中IL-10的表达，减轻内毒素血症小鼠肺、肝的损伤程度。特异性IgY具有治疗内毒素血症的潜力。
Aims: To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model.Methods and Results: Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity to LPS when subjected to an ELISA. Endotoxemia was induced in mice by intraperitoneal injection of LPS at a dose of 20 mg kg(-1) for measuring survival rate and 10 mg kg(-1) for cytokine measurement. The survival rate of mice treated with 200 mg kg(-1) specific IgY or 5 mg kg(-1) dexamethasone was 70% while none of the mice in the normal saline-treated group survived more than 7 days. Specific IgY significantly (P < 0 05) decreased tumour necrosis factor-alpha (TNF-alpha) level and increased interleukin-10 (IL-10) level in the serum of endotoxemia mice. Specific IgY had less of an effect on TNF-alpha than dexamthasone, while its effect on increasing IL-10 was stronger than dexamethasone. Haematoxylin and eosin-stained sections indicated that IgY attenuated the damage to the lung and liver observed in mice with endotoxemia.Conclusions: The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down-regulated TNF-alpha and up-regulated IL-10 in serum and attenuated the extent of damage to the lung and liver.Significance and Impact of the Study: The specific IgY has potential for the treatment of LPS-induced endotoxemia.