Ultraviolet light-induced inhibition of small nuclear RNA synthesis.
Ultraviolet light-induced inhibition of small nuclear RNA synthesis.
复制标题
紫外线诱导的小核 RNA 合成抑制。
DOI：
10.1002/jcp.1041380320
复制
发表时间：
1989
影响因子：
5.6
通讯作者：
Eliceiri,GL
中科院分区：
文献类型：
--
作者：
Eliceiri,BP;Choudhury,K;Scott,QO;Eliceiri,GL
Two apparently distinct types of inhibition of the synthesis of U1, U2, U3, U4, and U5 small nuclear RNA, induced by ultraviolet (UV) radiation, have been described before: immediate and delayed. Our present observations can be summarized as follows: a) neither the immediate nor the delayed inhibition appear to be mediated by the formation of cyclobutane pyrimidine dimers, since they were not prevented by photoreactivating light, in ICR 2A frog cells; b) the inhibition of U1 RNA synthesis, monitored in HeLa cells within the first few minutes after irradiation, extrapolated to a substantial suppression at time zero of postirradiation cell incubation, providing further support for the proposal that the immediate inhibition is a reaction separate from the delayed UV light‐induced inhibition of U1 RNA synthesis; c) the transition from the pattern of the immediate inhibition to that of the delayed inhibition (disappearance of the UV‐resistant fraction of U1 RNA synthesis and increased rate of inhibition) occurred gradually, without an apparent threshold, within the first 2 hr of incubation after irradiation; and d) the incident UV dose that resulted in a 37% level of residual U1 RNA synthesis (D37) during the delayed inhibition was about 7 J/m2, with an apparent UV dose threshold, and was about 60 J/m2for the immediate inhibition.
