SPECIFIC SUPPRESSION OF ANTIBODY-RESPONSE BY ANTIBODIES TO RECEPTORS
SPECIFIC SUPPRESSION OF ANTIBODY-RESPONSE BY ANTIBODIES TO RECEPTORS
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DOI：
10.1073/pnas.69.9.2701
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发表时间：
1972-01-01
影响因子：
11.1
通讯作者：
KOHLER, H
中科院分区：
文献类型：
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作者：
COSENZA, H;KOHLER, H
BALB/c骨髓瘤蛋白TEPC 15和MOPC 167与磷胆碱结合，并与肺炎球菌R36A的C-多糖部分沉淀。在A/HE小鼠体内培育的针对TEPC 15骨髓瘤的抗独特型抗体通过细胞释放对磷胆碱的抗体来防止斑块的形成，并抑制对磷胆碱体外和体内抗体反应的诱导。这种抑制是特异的，因为针对MOPC 167的非交叉反应独特型决定簇的抗体既不能阻止斑块的形成，也不能诱导抗体反应。此外，对绵羊红细胞的反应不受TEPC 15抗体的抑制。这些结果表明，抗磷胆碱抗体和磷胆碱细胞受体与TEPC 15骨髓瘤蛋白具有相似的抗原结合部位。因此，针对TEPC 15骨髓瘤蛋白的抗独特型抗体起到“抗受体”抗体的作用，因为它们专门阻止对磷胆碱的初始反应的诱导。有人认为，受体抗体可能参与了免疫反应的动态平衡。
Balb/c myeloma proteins, TEPC 15 and MOPC 167, bind phosphorylcholine and precipitate with the C-polysaccharide moiety of pneumococci R36A. Anti-idiotypic antibodies to TEPC 15 myeloma raised in A/He mice prevent plaque formation by cells releasing antibody to phosphorylcholine and inhibit induction of the antibody response to phosphorylcholinein vitroandin vivo. This suppression is specific since antibodies against non-crossreacting idiotypic determinants of MOPC 167 do not prevent either formation of plaques or induction of the antibody response. Furthermore, the response to sheep erythrocytes is not suppressed by antibodies to TEPC 15. These results indicate that antibodies to phosphorylcholine and cell receptors for phosphorylcholine share similar antigen-binding sites with TEPC 15 myeloma protein. Thus, anti-idiotypic antibodies directed against TEPC 15 myeloma protein function as “anti-receptor” antibodies since they specifically prevent induction of the primary response to phosphorylcholine. It is proposed that antibodies to receptors may be involved in the homeostasis of the immune response.