Cepheid GeneXpert MTB/RIF Assay for Mycobacterium tuberculosis Detection and Rifampin Resistance Identification in Patients with Substantial Clinical Indications of Tuberculosis and Smear-Negative Microscopy Results
Cepheid GeneXpert MTB/RIF Assay for Mycobacterium tuberculosis Detection and Rifampin Resistance Identification in Patients with Substantial Clinical Indications of Tuberculosis and Smear-Negative Microscopy Results
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DOI：
10.1128/jcm.00718-11
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发表时间：
2011-08-01
影响因子：
9.4
通讯作者：
Kanavaki, Sofia
中科院分区：
文献类型：
--
作者：
Ioannidis, Panayotis;Papaventsis, Dimitrios;Kanavaki, Sofia
使用具有结核病实质性临床指征的患者的镜检阴性和阳性肺和肺外标本评价GeneXpert MTB/RIF检测试剂盒。对于肺样本，灵敏度、特异性、阳性和阴性预测值分别为90.6%、94.3%、93.5%和91.7%，对于肺外样本，它们分别为100%、91.6%、50%和100%。对于显微镜下阴性标本，相应值分别为86.3%、93%、79%和95.6%。该检测试剂盒正确检出了除一份标本外的所有标本中的利福平耐药性，该标本含有混合种群。GeneXpert检测试剂盒可高效用于结核病诊断和痰涂片阴性样本中利福平耐药菌株的鉴定。
The GeneXpert MTB/RIF assay was evaluated with microscopically negative and positive pulmonary and extrapulmonary specimens from patients with substantial clinical indications for tuberculosis. For the pulmonary samples, the sensitivity, specificity, and positive and negative predictive values were 90.6%, 94.3%, 93.5%, and 91.7%, and for the extrapulmonary samples, they were 100%, 91.6%, 50%, and 100%, respectively. For microscopically negative specimens, the respective values were 86.3%, 93%, 79%, and 95.6%. The assay correctly detected rifampin resistance in all but one specimen, which harbored a mixed population. The GeneXpert assay was highly effective for tuberculosis diagnosis and identification of rifampin-resistant strains in smear-negative samples.