Rapid Semiautomated Subtyping of Influenza Virus Species during the 2009 Swine Origin Influenza A H1N1 Virus Epidemic in Milwaukee, Wisconsin
Rapid Semiautomated Subtyping of Influenza Virus Species during the 2009 Swine Origin Influenza A H1N1 Virus Epidemic in Milwaukee, Wisconsin
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DOI：
10.1128/jcm.00999-09
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发表时间：
2009-09-01
影响因子：
9.4
通讯作者：
Henrickson, Kelly J.
中科院分区：
文献类型：
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作者：
Bose, Michael E.;Beck, Eric T.;Henrickson, Kelly J.
2009 年春天，一种新型甲型 H1N1 流感病毒（猪源流感病毒 [S-OIV]）出现，并开始在威斯康星州密尔沃基市引发大规模疾病暴发。我们中西部呼吸道病毒计划实验室的小组开发了一种半自动实时多重逆转录 PCR 检测（季节性），采用 NucliSENS easyMAG 系统（北卡罗来纳州达勒姆的 BioMerieux）和 Raider 热循环仪（密歇根州安阿伯市的 HandyLab Inc.），对甲型流感病毒、乙型流感病毒和呼吸道合胞病毒 (RSV) 进行分型，并将甲型流感病毒分型到当前流行的 H1 和 H3 中亚型，以及鉴定 S-OIV H1 的类似测定。季节性和 H1 S-OIV 测定证明了检测的分析限
In the spring of 2009, a novel influenza A (H1N1) virus ( swine origin influenza virus [S-OIV]) emerged and began causing a large outbreak of illness in Milwaukee, WI. Our group at the Midwest Respiratory Virus Program laboratory developed a semiautomated real-time multiplex reverse transcription-PCR assay ( Seasonal), employing the NucliSENS easyMAG system (bioMerieux, Durham, NC) and a Raider thermocycler (HandyLab Inc., Ann Arbor, MI), that typed influenza A virus, influenza B virus, and respiratory syncytial virus (RSV) and subtyped influenza A virus into the currently circulating H1 and H3 subtypes, as well as a similar assay that identified H1 of S-OIV. The Seasonal and H1 S-OIV assays demonstrated analytical limits of detection of