Immunohistochemical detection of betaine-homocysteine S-methyltransferase in human, pig, and rat liver and kidney
Immunohistochemical detection of betaine-homocysteine S-methyltransferase in human, pig, and rat liver and kidney
复制标题
DOI：
10.1006/abbi.2001.2474
复制
发表时间：
2001-09-01
影响因子：
3.9
通讯作者：
Garrow, TA
中科院分区：
文献类型：
--
作者：
Delgado-Reyes, CV;Wallig, MA;Garrow, TA
甜菜碱-同型半胱氨酸S甲基转移酶(BHMT)在所有脊椎动物的肝脏中都有高水平的表达。它在灵长类动物和猪的肾脏中含量也很高，但在大鼠肾脏中含量很低，在单胃动物的其他主要器官中基本上不存在。我们最近通过酶活性和Western分析表明，猪肾BHMT只在皮质表达，在髓质中没有表达。通过免疫组织化学检测，我们在此报道在人、猪和大鼠肾中，BHMT在皮质近端小管中表达。人、猪和大鼠肝脏中BHMT的免疫组织化学染色表明，BHMT在肝细胞中高表达。染色模式与两个器官的胞浆表达一致。(C)2001年学术出版社。
Betaine-homocysteine S-methyltransferase (BHMT) has been shown to be expressed at high levels in the livers of all vertebrate species tested. It has also been shown to be abundant in primate and pig kidney but notably very low in rat kidney and essentially absent from the other major organs of monogastric animals. We recently showed by enzyme activity and Western analysis that pig kidney BHMT was only expressed in the cortex and was absent from the medulla. Using immunohistochemical detection, we report here that in human, pig, and rat kidney, BHMT is expressed in the proximal tubules of the cortex. Immunohistochemical staining for BHMT in human, pig, and rat liver indicate high expression in hepatocytes. The staining patterns are consistent with cytosolic expression in both organs. (C) 2001 Academic Press.