Analysis by comparative genomic hybridization of gastric cancer with peritoneal dissemination and/or positive peritoneal cytology
Analysis by comparative genomic hybridization of gastric cancer with peritoneal dissemination and/or positive peritoneal cytology
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DOI：
10.1016/j.cancergencyto.2005.01.007
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发表时间：
2005-08-01
影响因子：
--
通讯作者：
Kusano, M
中科院分区：
文献类型：
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作者：
Morohara, K;Nakao, K;Kusano, M
腹膜转移是影响胃癌预后的重要因素。虽然比较基因组杂交（CGH）在胃癌中的研究已有报道，但关于胃癌腹膜转移（P）和腹膜细胞学（CY）因素的研究报道较少。在这项研究中，我们分析了原发性肿瘤的染色体变化与激光显微切割分析和CGH相结合，试图发现未知的异常染色体区域。我们分析了34个原发性肿瘤，包括13个原发性肿瘤与腹膜转移（PI）和/或阳性腹膜细胞学（CY 1）使用激光显微切割和CGH的组合。将增益中的最小重叠区域分配给5 p14（46.2%），7q21.3（61.5%），7q31（46.2%）、7 q36（46.2%）、8 q23（53.8%）、15 q26（46.2%）、20 q12（61.5%）、20q13.1（53.8%）和20q13.2（53.8%）。最常发生丢失的最小区域是4 q34-q35（23.1%）和22q11.2（23.1%）。5 p14（P = 0.033）、7q21.3（P <0.0001）、7 q31（P = 0.013）、7 q36（P = 0.033）和22q11.2（P = 0.048）的微小区域在有和无PI和/或CY 1的原发性肿瘤之间存在显著差异。在本研究中，5 p14、7q21.3、7 q31和7 q36的获得/扩增和22q11.2的丢失在伴有腹膜转移和/或腹膜细胞学阳性的胃癌病例中是显著的。(c)2005年爱思唯尔公司All rights reserved.
Peritoneal metastasis is an important prognostic factor in cases of gastric cancer. Although studies on comparative genomic hybridization (CGH) in gastric cancer have been reported, there are few reports on the peritoneal metastasis (P) and peritoneal cytology (CY) factors in this cancer. In this study, we analyzed the chromosomal changes in the primary tumor with a combination of laser microdissection analysis and CGH in an attempt to detect the unknown abnormal chromosomal regions. We analyzed 34 primary tumors, including 13 primary tumors with peritoneal metastasis (PI) and/or positive peritoneal cytology (CY1) using a combination of laser microdissection and CGH. The minimal overlapping regions in gains were assigned to 5p14 (46.2 %), 7q21.3 (61.5 %), 7q31 (46.2 %), 7q36 (46.2 %), 8q23 (53.8 %), 15q26 (46.2 %), 20q12 (61.5 %), 20q13.1 (53.8 %), and 20q13.2 (53.8 %) in primary tumors with P1 and/or CY1. The minimal regions of losses that occurred most frequently were 4q34-q35 (23.1 %) and 22q11.2 (23.1 %). There were significant differences in the minimal regions of 5p14 (P = 0.033), 7q21.3 (P < 0.000 1), 7q31 (P = 0.0 13), 7q36 (P = 0.033), and 22q11.2 (P = 0.048) between primary tumors with and without PI and/or CY1. In this study, gain/amplification of 5p 14, 7q21.3, 7q31, and 7q36, and loss of 22q 11.2 were significant in gastfic cancer cases with peritoneal dissemination and/or positive peritoneal cytology. (c) 2005 Elsevier Inc. All rights reserved.