Rhythmic spontaneous contractions in the rat proximal colon.
Rhythmic spontaneous contractions in the rat proximal colon.
复制标题
大鼠近端结肠的节律性自发收缩。
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Takaki
中科院分区：
文献类型：
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作者：
S. Yoneda;M. Kadowaki;S. Sugimori;F. Sekiguchi;S. Sunano;H. Fukui;M. Takaki
C-kit免疫反应细胞被称为Cajal间质细胞（ICC），它们产生胃肠道的起搏活动。最近在豚鼠近端结肠中发现了大量与c-kit免疫反应阳性细胞相对应的特殊平滑肌细胞。我们了解到，大鼠近端结肠表现出河豚毒素不敏感的规律性节律性自发收缩（RSC），并假设RSC由ICC产生和/或调节。为了证明我们的假设，我们研究了纯合子Ws/Ws突变大鼠中是否不存在RSC，因为缺乏沿着环行肌（ICC（SM））和肌间神经丛（ICC（MY））粘膜下表面的c-kit阳性ICC。与我们的假设相反，我们发现RSCs仍然存在于Ws/Ws突变大鼠的近端结肠中。最近的一项研究报告，c-kit阴性ICC（SM）仍然存在于Ws/Ws突变大鼠。综上所述，RSC可以由大鼠近端结肠中的c-kit阴性ICC（SM）产生。用环匹阿尼酸（CPA）（10（-6）M）或毒胡萝卜素（10（-6）M）阻断肌浆网Ca（2+）-ATP酶可增加RSC的频率。CPA对Ws/Ws突变大鼠RSCs频率的增加作用比+/+大鼠更明显。我们的结论是，c-kit阴性ICC（SM）和其他突变受损的c-kit阳性ICC（MY）和ICC（SM）之间的功能协调可能需要适度调节自发活动的频率。
C-kit immunoreactive cells are known to be interstitial cells of Cajal (ICCs), and they generate pacemaker activity of the gastrointestinal tract. Recently a large number of special smooth muscle cells corresponding to c-kit immunoreactive cells were found in the proximal colon of the guinea pig. We learned that the rat proximal colon showed tetrodotoxin-insensitive regular rhythmic spontaneous contractions (RSCs) and hypothesized that RSCs are generated and/or regulated by ICCs. To prove our hypothesis, we investigated whether RSCs are absent in homozygous Ws/Ws mutant rats, since c-kit positive ICCs along the submucosal surface of the circular muscle (ICC(SM)) and myenteric plexus (ICC(MY)) are lacking. In contrast to our hypothesis, we found that RSCs were still present in the proximal colon of the Ws/Ws mutant rats. A recent study has reported that c-kit negative ICC(SM) remains in Ws/Ws mutant rats. Taken together, RSCs may be generated by c-kit negative ICC(SM) in the rat proximal colon. The blockade of sarcoplasmic reticulum Ca(2+)-ATPase by cyclopiazonic acid (CPA) (10(-6)M) or by thapsigargin (10(-6)M) increased the frequency of RSCs. The increasing effects of CPA on the frequency of RSCs were more prominent in Ws/Ws mutant rats than in +/+ rats. We concluded that the functional coordination between c-kit negative ICC(SM) and other mutationally impaired c-kit positive ICC(MY) and ICC(SM) may be required for moderate regulation in the frequency of spontaneous activity.
毒胡萝卜素和 2,5-t-丁基对苯二酚可抑制 L 型钙通道活性，但环吡嗪酸不会抑制 L 型钙通道活性。
DOI：
10.1042/bj3020147
发表时间：
1994
期刊：
The Biochemical journal
影响因子：
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作者：
Nelson,EJ;Li,CC;Bangalore,R;Benson,T;Kass,RS;Hinkle,PM
通讯作者：
Hinkle,PM