Plasma cytokines as markers of aseptic prosthesis loosening
Plasma cytokines as markers of aseptic prosthesis loosening
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DOI：
10.1097/01.blo.0000229365.57985.96
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发表时间：
2006-12-01
影响因子：
4.2
通讯作者：
Jukic, Irena
中科院分区：
文献类型：
--
作者：
Hundric-Haspl, Zeljka;Pecina, Marko;Jukic, Irena
促炎细胞因子IL-1 β、IL-8和TNF-α在大关节假体无菌性松动期间的骨吸收过程中起主要作用。这些细胞因子在无菌性松动的骨吸收过程中局部分泌，可进入外周循环。外周循环中IL-1 β、IL-8和TNF-α浓度升高可能提示无菌性松动。我们确定是否可以通过血浆中细胞因子的存在来验证骨吸收。我们招募了50名无菌性假体松动患者，50名稳定假体患者，50名骨关节炎患者和50名健康个体。通过ELISA试验测定血浆中的细胞因子水平。假体松动的患者血浆中（IL-1 β，3.7 ± 5.5 pg/mL; IL-8，14.7 ± 9 pg/mL; TNF-α，32.7 ± 32.4 pg/mL）（IL-1 β，1.5 +/- 2 pg/mL; IL-8，8.1 +/0 4.7 pg/mL; TNF-α，22.9 +/- 18.7 pg/mL），骨关节炎患者（IL-1 β，0.7 +/- 1.1 pg/mL; IL-8，5.8 +/- 3.8 pg/mL; TNF-α，9.8 +/- 7.7 pg/mL）和健康个体（IL-1 β，0.7 +/- 1.1 pg/mL; IL-8，4.2 +/- 1.3 pg/mL; TNF-α，3.9 +/- 3.9 pg/mL）。我们的数据表明，促炎细胞因子的血浆水平升高可能是有用的标记骨吸收的实验室诊断假体松动。
The proinflammatory cytokines IL-1 beta, IL-8, and TNF-alpha play a major role in the process of bone resorption during aseptic loosening of large joint prostheses. These cytokines secreted locally during bone resorption in aseptic loosening may enter peripheral circulation. Increased concentration of IL-1 beta, IL-8, and TNF-alpha in peripheral circulation may indicate aseptic loosening. We determined whether bone resorption could be verified by cytokine presence in plasma. We recruited 50 patients with aseptic prosthesis loosening, 50 with stable prostheses, 50 with osteoarthritis, and 50 healthy individuals. Cytokine levels were determined in plasma by ELISA tests. Patients with prosthesis loosening had higher plasma levels (IL-1 beta, 3.7 +/- 5.5 pg/mL; IL-8,14.7 +/- 9 pg/mL; TNF-alpha, 32.7 +/- 32.4 pg/mL) than patients with stable prostheses (IL-1 beta, 1.5 +/- 2 pg/mL; IL-8, 8.1 +/0 4.7 pg/mL; TNF-alpha, 22.9 +/- 18.7 pg/mL), patients with osteoarthritis (IL-1 beta, 0.7 +/- 1.1 pg/mL; IL-8, 5.8 +/- 3.8 pg/mL; TNF-alpha, 9.8 +/- 7.7 pg/mL) and healthy individuals (IL-1 beta, 0.7 +/- 1.1 pg/mL; IL-8, 4.2 +/- 1.3 pg/mL; TNF-alpha, 3.9 +/- 3.9 pg/mL). Our data suggest elevated plasma levels of proinflammatory cytokines may be useful as markers of bone resorption in the laboratory diagnosis of prosthesis loosening.