Effect of baicalin on GLUT4 expression and glucose uptake in myotubes of rats
Effect of baicalin on GLUT4 expression and glucose uptake in myotubes of rats
复制标题
黄芩苷对大鼠肌管GLUT4表达及葡萄糖摄取的影响
DOI：
10.1016/j.lfs.2018.01.022
复制
发表时间：
2018-03-01
期刊：
影响因子：
6.1
通讯作者：
Bo, Ping
中科院分区：
文献类型：
--
作者：
Fang, Penghua;Yu, Mei;Bo, Ping
目的：虽然黄芩苷可以减轻肥胖诱导的胰岛素抵抗，但其对葡萄糖摄取的作用机制尚不清楚。本研究的目的是调查黄芩苷是否可以促进L 6肌管的葡萄糖摄取并探讨其信号机制。材料和方法：本研究用100、200、400 μ M黄芩苷处理L 6肌管6 h、12 h和24 h。结果：黄芩苷能提高L 6细胞肌管中GLUT 4、PGC-1 α、pP 38 MAPK、pAKT和pAS 160的含量以及GLUT 4和PGC-1 α mRNA的表达。黄芩苷诱导的L 6肌管葡萄糖代谢改变可被P38 MAPK或AKT抑制剂阻断。结论：黄芩苷通过AKT/AS 160/GLUT 4和P38 MAPK/PGC 1 α/GLUT 4通路治疗高血糖症具有广阔的应用前景。
Aims: Although baicalin could attenuate obesity-induced insulin resistance, the detailed mechanism of baicalin on glucose uptake has not been sufficiently explored as yet. The aim of this study was to survey if baicalin might facilitate glucose uptake and to explore its signal mechanisms in L6 myotubes.Materials and methods: L6 myotubes were treated with 100, 200, 400 mu M baicalin for 6 h, 12 h and 24 h in this study. Then 2-NBDG and insulin signal protein levels in myotubes of L6 cells were examined.Key findings: We discovered that administration of baicalin enhanced GLUT4, PGC-1 alpha, pP38MAPK, pAKT and pAS160 contents, as well as GLUT4 mRNA and PGC-1 alpha mRNA levels in L6 myotubes. The beneficial metabolic changes elicited by baicalin were abrogated in myotubes of L6 by P38MAPK or AKT inhibitors.Significance: These results suggest that baicalin promoted glucose uptake in myotubes by differential regulation on P38MAPK and AKT activity. In conclusion, these data provide insight that baicalin is a powerful and promising agent for the treament of hyperglycemia via AKT/AS160/GLUT4 and P38MAPK/PGC1 alpha/GLUT4 pathway.