Proximal tubular function in transgenic mice overexpressing atrial natriuretic factor.
Proximal tubular function in transgenic mice overexpressing atrial natriuretic factor.
复制标题
过度表达心房钠尿因子的转基因小鼠的近端肾小管功能。
DOI：
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发表时间：
1994
影响因子：
2.1
通讯作者：
A. Veress
中科院分区：
文献类型：
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作者：
H. Sonnenberg;U. Honrath;C. Chong;L. Field;A. Veress
一种转基因小鼠模型，其中心房钠尿因子（ANF）表达针对肝脏，用于研究肾内对长期升高的激素水平的调节。这些动物被命名为 TTR-ANF，其特点是与非转基因动物相比，动脉血压降低，但钠排泄相似。近端肾小管微穿刺得到以下结果：单肾单位滤过率 = 12.7 +/- 1.1 vs. 15.6 +/- 1.9 nL/min（TTR-ANF 与非转基因，ns）；菊粉的近端肾小管液体/血浆浓度比 = 1.93 +/- 0.09 vs. 1.97 +/- 0.15 (ns)；钠重吸收分数 = 45.5 +/- 2.8 vs. 46.0 +/- 3.8% (ns)；氯离子重吸收分数 = 33.6 +/- 3.3 vs. 32.4 +/- 4.1% (ns)。这些数据表明，血浆 ANF 浓度的终生升高与单肾单位滤过率和近端肾小管功能的显着改变无关。我们的结论是，位于近曲小管下游的代偿性抗尿钠排泄机制可以预防 ANF 尿钠排泄。
A transgenic mouse model in which atrial natriuretic factor (ANF) expression is targeted to the liver was used to study intrarenal adjustments to the chronically elevated hormone level. Such animals, designated TTR-ANF, are characterized by reduced arterial blood pressure but similar sodium excretion compared with nontransgenic siblings. Proximal tubular micropuncture gave the following results: single-nephron filtration rate = 12.7 +/- 1.1 vs. 15.6 +/- 1.9 nL/min (TTR-ANF versus nontransgenic, ns); end-proximal tubular fluid/plasma concentration ratio of inulin = 1.93 +/- 0.09 vs. 1.97 +/- 0.15 (ns); fractional reabsorption of sodium = 45.5 +/- 2.8 vs. 46.0 +/- 3.8% (ns); fractional reabsorption of chloride = 33.6 +/- 3.3 vs. 32.4 +/- 4.1% (ns). These data indicate that life-long elevation of plasma ANF concentration was not associated with significant alteration in single-nephron filtration rate and proximal tubular function. We conclude that compensatory antinatriuretic mechanisms, localized downstream from the proximal tubule, can prevent ANF natriuresis.