Development of a protocol for maintaining viability while shipping organoid-derived retinal tissue.
Development of a protocol for maintaining viability while shipping organoid-derived retinal tissue.
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DOI：
10.1002/term.2997
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发表时间：
2020-02
影响因子：
3.3
通讯作者：
Nasonkin IO
中科院分区：
文献类型：
--
作者：
Singh RK;Winkler P;Binette F;Glickman RD;Seiler M;Petersen-Jones SM;Nasonkin IO
视网膜类器官技术能够从人类多能干细胞（hPSC）中产生取之不尽的三维视网膜组织，用于再生医学应用。类器官衍生的视网膜组织和可移植的人胎儿视网膜的高度相似性提供了在相关动物模型中评估和建模视网膜组织替代策略的机会，以努力开发功能性视网膜贴片来恢复由视网膜变性引起的深度失明患者的视力。由于这种非常有前途的方法的复杂性，需要专门的干细胞和移植技术，视网膜组织衍生和移植的任务经常在地理上遥远的团队之间分配。将脆弱且易腐烂的神经组织如视网膜递送到手术部位需要可靠的运输协议，并且还需要具有适当的损伤报告机制的受控温度条件，以防止将运输中受损的组织移植到昂贵的动物模型中。我们已经开发了一个强大的隔夜组织运输协议，提供可靠的温度控制，实时监控运输条件和包装的物理位置，并在交付时报告损坏情况。这允许在长距离上运输有活力的（移植能力）hPSC衍生的视网膜组织，从而使来自全国不同地区的干细胞和手术团队能够共同工作，并最大限度地成功植入类器官衍生的视网膜组织。虽然该方案是为动物模型的临床前体内研究开发的，但它在未来可能可用于临床移植，并将有助于开发恢复视网膜变性患者视力的临床方案。
Retinal organoid technology enables generation of an inexhaustible supply of three-dimensional retinal tissue from human pluripotent stem cells (hPSCs) for regenerative medicine applications. The high similarity of organoid-derived retinal tissue and transplantable human fetal retina provides an opportunity for evaluating and modeling retinal tissue replacement strategies in relevant animal models in the effort to develop a functional retinal patch to restore vision in patients with profound blindness caused by retinal degeneration. Because of the complexity of this very promising approach requiring specialized stem cell and grafting techniques, the tasks of retinal tissue derivation and transplantation are frequently split between geographically distant teams. Delivery of delicate and perishable neural tissue such as retina to the surgical sites requires a reliable shipping protocol and also controlled temperature conditions with damage-reporting mechanisms in place to prevent transplantation of tissue damaged in transit into expensive animal models. We have developed a robust overnight tissue shipping protocol providing reliable temperature control, live monitoring of the shipment conditions and physical location of the package, and damage reporting at the time of delivery. This allows for shipping of viable (transplantation-competent) hPSC-derived retinal tissue over large distances, thus enabling stem cell and surgical teams from different parts of the country to work together and maximize successful engraftment of organoid-derived retinal tissue. Although this protocol was developed for preclinical in vivo studies in animal models, it is potentially translatable for clinical transplantation in the future and will contribute to developing clinical protocols for restoring vision in patients with retinal degeneration.
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影响因子：
4.2
作者：
Radtke, Norman D.;Aramant, Robert B.;Seiler, Magdalene J.
通讯作者：
Seiler, Magdalene J.
影响因子：
16.6
作者：
Zhong, Xiufeng;Gutierrez, Christian;Xue, Tian;Hampton, Christopher;Vergara, M. Natalia;Cao, Li-Hui;Peters, Ann;Park, Tea Soon;Zambidis, Elias T.;Meyer, Jason S.;Gamm, David M.;Yau, King-Wai;Canto-Soler, M. Valeria
通讯作者：
Canto-Soler, M. Valeria
DOI：
10.1007/s00417-014-2638-y
发表时间：
2014-07-01
影响因子：
2.7
作者：
Seiler, Magdalene J.;Aramant, Robert B.;Keirstead, Hans S.
通讯作者：
Keirstead, Hans S.
影响因子：
17.8
作者：
Seiler MJ;Aramant RB
通讯作者：
Aramant RB
影响因子：
3.4
作者：
Lin B;McLelland BT;Mathur A;Aramant RB;Seiler MJ
通讯作者：
Seiler MJ