Somatosensory cortical neuronal population activity across states of anaesthesia
Somatosensory cortical neuronal population activity across states of anaesthesia
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DOI：
10.1046/j.0953-816x.2002.01898.x
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发表时间：
2002-02-01
影响因子：
3.4
通讯作者：
Diamond, ME
中科院分区：
文献类型：
--
作者：
Erchova, IA;Lebedev, MA;Diamond, ME
进行了实验，以了解与不同程度的麻醉相关的感觉皮层处理的变化。传统上，这个问题是通过研究单个神经元来解决的。由于状态变化可能会影响神经元之间的关系，本实验进行了调查的空间和时间分布在大脑皮层的放电模式。使用5 × 5或10 × 10的微电极阵列，自发和刺激诱发的活动的多神经元簇记录从大鼠体感“桶”皮层（晶须表示）在轻手术阶段的氨基甲酸乙酯麻醉，并在两个补充剂量的氨基甲酸乙酯，导致中间和深层次的麻醉。在所有麻醉深度下，单个电极上自发发生的动作电位往往聚集成“爆发”。随着麻醉深度的增加，爆发变得更加突出和有节奏，并且在皮质桶柱之间越来越同步。爆发频率降低，爆发外出现的尖峰更少，导致整体自发放电率降低。个别胡须从事的皮质领土收缩与麻醉深度的增加，导致空间隔离的胡须表示。在麻醉的所有阶段，当接近爆发发作时，胡须刺激产生最大的皮层反应。这些观察结果表明，正在进行的自发活动调制的感觉反应特性，并使周边触觉信息访问的皮质领土的大小是由爆发周期的阶段。被认为是可能的意义，在清醒的大鼠在乌拉坦麻醉皮质处理过程中遇到的周期性皮质反应。
Experiments were carried out to learn about changes in sensory cortical processing associated with different levels of anaesthesia. Traditionally this question has been addressed by studying single neurons. Because state changes are likely to influence the relationships between neurons, the present experiments were undertaken to investigate the spatial and temporal firing patterns distributed across cortex. Using 5 x 5 or 10 x 10 microelectrode arrays, spontaneous and stimulus-evoked activity of multineuron clusters was recorded from rat somatosensory 'barrel' cortex (the whisker representation) during a light surgical stage of urethane anaesthesia, and after two supplemental doses of urethane which led to intermediate and deep levels of anaesthesia. At all depths of anaesthesia, spontaneously occurring action potentials at a single electrode tended to be clustered into 'bursts.' With increasing anaesthetic depth, bursts became more prominent and rhythmic, and increasingly synchronized between cortical barrel-columns. Burst frequency decreased and fewer spikes occurred outside bursts, leading to a decrease in the overall spontaneous firing rate. The cortical territory engaged by individual whiskers contracted with increasing depth of anaesthesia, leading to the spatial segregation of whisker representations. At all stages of anaesthesia, whisker stimulation produced the maximal cortical response when delivered close to burst onset. These observations show that ongoing spontaneous activity modulates sensory response properties and makes peripheral tactile information accessible to a cortical territory whose size is determined by the phase of burst cycle. The possible significance of the cyclic cortical responsiveness encountered during urethane anaesthesia to cortical processing in awake rats is considered.