Melatonin ameliorates bisphenol A-induced biochemical toxicity in testicular mitochondria of mouse
Melatonin ameliorates bisphenol A-induced biochemical toxicity in testicular mitochondria of mouse
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DOI：
10.1016/j.fct.2011.07.062
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发表时间：
2011-11-01
影响因子：
4.3
通讯作者：
Raisuddin, Sheikh
中科院分区：
文献类型：
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作者：
Anjum, Sameya;Rahman, Shakilur;Raisuddin, Sheikh
双酚A（BPA）是聚碳酸酯塑料的单体，用于制造塑料婴儿奶瓶和食品罐衬里。它具有内分泌干扰潜力，对哺乳动物细胞具有毒性和雌激素效应。我们研究了BPA诱导的瑞士白化病小鼠睾丸线粒体标记酶的扰动和褪黑素的改善作用。小鼠经口暴露于标准剂量BPA（10 mg/kg体重）14天后，其标志性线粒体酶（如琥珀酸脱氢酶、苹果酸脱氢酶、异柠檬酸脱氢酶、单胺氧化酶和NADH脱氢酶）的活性降低。此外，它还影响抗氧化酶如超氧化物歧化酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶的活性。BPA还引起线粒体脂质过氧化（LPO）和还原型谷胱甘肽（GSH）含量下降。伴随褪黑激素给药（10 mg/kg体重;腹膜内给药14天）降低线粒体脂质过氧化。它还恢复了线粒体标记酶的活性，并改善了线粒体酶和非酶抗氧化剂的减少。这些结果表明，褪黑激素在改善BPA诱导的线粒体毒性中具有潜在的作用，这种保护作用是由于其抗氧化特性或直接的自由基清除活性。(C)2011爱思唯尔有限公司保留所有权利。
Bisphenol A (BPA) is a monomer of polycarbonate plastic used to manufacture plastic baby bottles and lining of food cans. It has endocrine-disrupting potential and exerts both toxic and estrogenic effects on mammalian cells. We studied BPA-induced perturbation of mitochondrial marker enzymes in testes of Swiss albino mice and its amelioration by melatonin. Mice exposed to standardized dose of BPA (10 mg/kg body weight) orally for 14 days showed decrease in activities of marker mitochondrial enzymes such as succinate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, monoamine oxidase and NADH dehydrogenase. Besides, it also affected activities of antioxidant enzymes such as superoxide dismutase, glutathione reductase and glutathione peroxidase. BPA also caused lipid peroxidation (LPO) and decrease in reduced glutathione (GSH) content of mitochondria. Concomitant melatonin administration (10 mg/kg body weight; intraperitoneally for 14 days) lowered mitochondrial lipid peroxidation. It also restored the activity of mitochondrial marker enzymes and ameliorated decreased enzymatic and non-enzymatic antioxidants of mitochondria. These results demonstrate that melatonin has a potential role in ameliorating BPA-induced mitochondrial toxicity and the protection is due to its antioxidant property or by the direct free radical scavenging activity. (C) 2011 Elsevier Ltd. All rights reserved.