Light stimulates the rapid formation of inositol trisphosphate in squid retinas.
Light stimulates the rapid formation of inositol trisphosphate in squid retinas.
复制标题
光刺激鱿鱼视网膜中三磷酸肌醇的快速形成。
DOI：
10.1042/bj2400929
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Fein,A
中科院分区：
文献类型：
--
作者：
Szuts,EZ;Wood,SF;Reid,MS;Fein,A
为了验证这一假设，即肌醇三磷酸（InsP 3）介导的适应和兴奋的无脊椎动物的光感受器，我们测量了其形成的快速时间尺度在鱿鱼视网膜。对于鱿鱼，兴奋和适应分别发生在0.1和1- 2s内。我们可以在明亮闪光的200毫秒内检测到InsP 3的升高。这种增加比暗基础水平高约240%，并且在闪光后保持至少2分钟。增加可能发生在光感受器，这是鱿鱼视网膜中唯一的神经元。h.p.l.c.分析表明光调节异构体是Ins（1，4，5）P3，其通过磷脂酰肌醇二磷酸（PtdInsP 2）的水解形成。
To test the hypothesis that inositol trisphosphate (InsP3) mediates adaptation and excitation in invertebrate photoreceptors, we measured its formation on a rapid time scale in squid retinas. For squid, excitation and adaption occurs within 0.1 and 1-2 s respectively. We could detect an elevation in InsP3 within 200 ms of a bright flash. This increase is about 240% over dark basal levels and is maintained for at least 2 min after a flash. The increase probably occurs in the photoreceptors, which are the only neurons in squid retinas. Analysis by h.p.l.c. indicates that the light-regulated isomer is Ins(1,4,5)P3, which is formed by the hydrolysis of phosphatidylinositol bisphosphate (PtdInsP2).