Dual desorption electrospray ionization-laser desorption ionization mass spectrometry on a common nanoporous alumina platform for enhanced shotgun proteomic analysis.
Dual desorption electrospray ionization-laser desorption ionization mass spectrometry on a common nanoporous alumina platform for enhanced shotgun proteomic analysis.
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在通用纳米多孔氧化铝平台上进行双解吸电喷雾电离-激光解吸电离质谱分析，用于增强鸟枪法蛋白质组分析。
DOI：
10.1021/ac801586r
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发表时间：
2008
影响因子：
7.4
通讯作者：
Knapp,DanielR
中科院分区：
文献类型：
--
作者：
Nayak,Ranu;Liu,Jian;Sen,AshisK;Knapp,DanielR
使用解吸电喷雾电离（DESI）和激光解吸电离（LDI）将金涂覆的纳米多孔氧化铝表面用于双电离模式质谱分析。将纳米多孔氧化铝表面的DESI和LDI质谱（MS）与传统的电喷雾电离（ESI）质谱和基质辅助激光解吸电离（MALDI）质谱进行了比较，用于蛋白质的胰蛋白酶解产物的分析。DESI和LDI的组合使用提供了比单独使用任一种方法更大的肽覆盖率，并且与双MALDI和ESI的肽覆盖率相当。这种双电离技术使用具有相同样品点的共同平台，证明了用于改进鸟枪蛋白质组学分析的潜在时间和成本效益工具。
A gold coated nanoporous alumina surface was used for dual ionization mode mass spectrometric analysis using desorption electrospray ionization (DESI) and laser desorption ionization (LDI). DESI and LDI mass spectrometry (MS) from the nanoporous alumina surface were compared with conventional electrospray ionization (ESI) mass spectrometry and matrix assisted laser desorption ionization (MALDI) for analysis of tryptic digests of proteins. Combined use of DESI and LDI offer greater peptide coverage than either method alone and comparable peptide coverage as with dual MALDI and ESI. This dual ionization technique using a common platform with same sample spot demonstrates a potential time and cost-effective tool for improved shotgun proteomic analysis.