Abundant nuclear copies of mitochondrial origin (NUMTs) in the Aedes aegypti genome
Abundant nuclear copies of mitochondrial origin (NUMTs) in the Aedes aegypti genome
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DOI：
10.1111/j.1365-2583.2009.00925.x
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发表时间：
2009-12-01
影响因子：
2.6
通讯作者：
Bernhardt, S. A.
中科院分区：
文献类型：
--
作者：
Black, W. C.;Bernhardt, S. A.
使用PCR在42 ℃退火温度下扩增埃及伊蚊线粒体NADH脱氢酶亚基4基因（ND 4）的一部分。个体蚊子的扩增片段与ND 4相似，但含有多个分离位点。我们怀疑线粒体起源的核拷贝（NUMTs）存在于Ae。埃及人基因组在VectorBase中搜索整个Ae。埃及伊蚊线粒体基因组鉴定出233个NUMT，在145个超重叠群中包含110 178 bp。在0.080 bp/kb的密度下，这代表昆虫基因组中NUMT的第二高密度和双翅目中的最高密度。侧翼序列分析表明，Ae.埃及伊蚊NUMT是通过线粒体DNA从受损线粒体中泄漏，随后发生断裂和非同源重组而产生的，而不是通过重复过程，如转座或分子驱动。
A portion of the Aedes aegypti mitochondrial NADH dehydrogenase subunit 4 gene (ND4) was amplified using PCR with a 42 degrees C annealing temperature. Amplified fragments from individual mosquitoes were similar to ND4 but contained multiple segregating sites. We suspected that nuclear copies of mitochondrial origin (NUMTs) exist in the Ae. aegypti genome. A BlastN search in VectorBase with the entire Ae. aegypti mitochondrial genome identified 233 NUMTs comprising 110 178 bp in 145 supercontigs. At a density of 0.080 bp/kb, this represents the second highest density of NUMTs in an insect genome and the highest in Diptera. Analyses of flanking sequences suggested that Ae. aegypti NUMTs arise through mtDNA leakage from damaged mitochondria followed by breakage and nonhomologous recombination, rather than through duplicative processes such as transposition or molecular drive.