Evidence for non-receptor odor discrimination using neuroblastoma cells as a model for olfactory cells
Evidence for non-receptor odor discrimination using neuroblastoma cells as a model for olfactory cells
复制标题
使用神经母细胞瘤细胞作为嗅觉细胞模型进行非受体气味辨别的证据
DOI：
10.1016/0006-8993(85)91416-7
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
K. Kurihara
中科院分区：
文献类型：
--
作者：
M. Kashiwayanagi;K. Kurihara
小鼠神经母细胞瘤细胞（N-18克隆），这是独立的嗅觉细胞，被去极化的20气味检查，这表明特定的蛋白质是不需要接收气味。使用N-18细胞检查非受体介导的气味辨别的机制。用不同的荧光探针测量了由吸附气味物质引起的细胞膜流动性的变化，这些荧光探针监测膜的不同深度和不同阶段的流动性。用这些染料监测的膜流动性变化的配置文件是不同的，从一个物种的气味剂到另一个，这表明具有不同气味的气味剂被吸附在膜中的不同位点。硬脂酸和胆固醇的外源性应用所带来的细胞膜的脂质组成的改变导致各种气味的反应（去极化的幅度）的修改。的程度和方向（增加或减少）的变化的反应大大不同种的气味。提出了以下关于气味辨别的机制。每个嗅觉细胞的膜组成被假定为细胞与细胞不同。膜中脂质和蛋白质的不同组合为气味剂提供了不同的吸附位点。在许多嗅觉细胞中响应于气味剂的膜电位变化的相对量是气味剂种类的特征。细胞水平上的响应曲线决定了气味的质量。
The mouse neuroblastoma cell (N-18 clone), which is independent of an olfactory cell, was depolarized by 20 odorants examined, suggesting that specific proteins are not required for reception of odorants. The mechanism of non-receptor-mediated odor discrimination was examined using the N-18 cell. Changes in the membrane fluidity of the cell induced by adsorption of odorants were measured with various fluorescence probes, which monitor the fluidity at the different depth and in the different phase of the membrane. The profiles of the membrane fluidity changes monitored with these dyes were different from one species of odorants to another, suggesting that odorants having different odors are adsorbed at different sites in the membranes. The alteration of the lipid composition of the cell membrane brought about by exogenous application of stearic acid and cholesterol led to modification of the responses (magnitude of depolarization) to various odorants. The extent and direction (increase or decrease) of changes in the responses greatly varied among species of odorants. The following mechanism on odor discrimination was proposed. A membrane composition of each olfactory cell is postulated to be different from cell to cell. Different combinations of lipids and proteins in the membranes provide different adsorption sites for odorants. Relative amounts of the membrane potential changes in many olfactory cells in response to an odorant are characteristic of the species of the odorant. The response profiles at the cell level determine the quality of the odor.