Comparison of different decellularization procedures of porcine heart valves
Comparison of different decellularization procedures of porcine heart valves
复制标题
DOI：
10.1177/039139880302600508
复制
发表时间：
2003-05-01
影响因子：
1.7
通讯作者：
Simon, P
中科院分区：
文献类型：
--
作者：
Kasimir, MT;Rieder, E;Simon, P
背景：组织工程心脏瓣膜应克服传统人工瓣膜的缺点。在这项研究中，我们测试了不同的脱细胞程序，其潜在的细胞去除和他们的能力，以保持matrix.Methods：标本的猪主动脉和肺根处理胰蛋白酶或十二烷基硫酸钠（SDS）或Triton-X100（（R））和脱氧胆酸钠的浓度范围。组织样品，然后处理扫描电子显微镜和激光扫描microscopic.Results：胰蛋白酶只实现不完全脱细胞化，并造成严重的结构改变的矩阵。相反，SDS完全去除细胞，但引起强烈的结构改变。Triton-X100（（R））和脱氧胆酸钠的治疗实现了完全脱细胞和保存matrix structure.Conclusion：脱细胞技术的效率和基质的保存是高度可变的，在我们的研究与Triton-X100（（R））和脱氧胆酸钠达到最好。
Background: Tissue engineering of heart valves should avoid the disadvantages of conventional prostheses. In this study we tested different decellularization procedures for their potential of cell removal and their ability to preserve the matrix.Methods: Specimens of porcine aortic and pulmonary roots were treated with either trypsin or sodium-dodecyl-sulfate (SDS) or Triton-X100((R)) and sodium-deoxycholate with a range of concentrations. Tissue samples were then processed for scanning electron microscopy and laser scanning microscopy.Results: Trypsin achieved only incomplete decellularization and caused severe structural alterations of the matrix. In contrast SDS removed cells completely but caused strong structural alterations. Treatment with Triton-X100((R)) and sodium-deoxycholate achieved both complete decellularization and preservation of the matrix structure.Conclusion: Techniques of decellularization are highly variable in efficiency and matrix preservation and was best achieved in our study with Triton-X100((R)) and sodium deoxycholate.