A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms
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DOI：
10.1080/20002297.2020.1771071
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发表时间：
2020-01-01
影响因子：
4.5
通讯作者：
Ramage, Gordon
中科院分区：
文献类型：
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作者：
Arias, Lais Salomao;Brown, Jason L.;Ramage, Gordon
背景能够提高现有抗菌药物的疗效的新颖和新的治疗策略是一个有吸引力的命题，以满足society.Objective目的本研究旨在评估咪康唑（MCZ）纳米载体系统的增效作用，纳入氧化铁纳米颗粒（IONP）和壳聚糖（CS）（IONP CS MCZ）。这是测试三个代表性的复杂的王国间的口腔生物膜模型（龋齿，义齿和牙龈炎）。材料和方法的最小抑菌浓度（MIC）的IONP-CS-MCZ对不同的白色念珠菌菌株进行了测定，以及对所有代表的细菌物种，在三个生物膜模型内形成。结果与单用MCZ相比，IONPs-CS-MCZ的MIC值更高。与未处理的生物膜相比，IONPs-CS-MCZ还促进代表性生物膜的CFU数量、生物量和代谢活性的减少，以及改变生物膜超微结构。IONPs-CS-MCZ影响了组成，并降低了三种评价生物膜中存在的大多数微生物的CFE。特别是，在所有三种模型中，链球菌在生物膜组成中的比例降低，而梭杆菌属。结论在体外实验中，IONPs-CS-MCZ纳米载体对三种致病性口腔生物膜模型均有效，表明其有可能干扰多微生物菌群中真菌和细菌细胞之间的协同作用。
Background Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society.Objective This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative complex interkingdom oral biofilm models (caries, denture and gingivitis).Materials and methods The planktonic and sessile minimum inhibitory concentrations (MICs) of IONPs-CS-MCZ against different Candida albicans strains were determined, as well as against all represented bacterial species that formed within the three biofilm models. Biofilms were treated for 24 hours with the IONPs-CS nanocarrier system containing MCZ at 64 mg/L, and characterized using a range of bioassays for quantitative and qualitative assessment.Results MIC results generally showed that IONPs-CS-MCZ was more effective than MCZ alone. IONPs-CS-MCZ also promoted reductions in the number of CFUs, biomass and metabolic activity of the representative biofilms, as well as altering biofilm ultrastructure when compared to untreated biofilms. IONPs-CS-MCZ affected the composition and reduced the CFEs for most of the microorganisms present in the three evaluated biofilms. In particular, the proportion of streptococci in the biofilm composition were reduced in all three models, whilst Fusobacterium spp. percentage reduced in the gingivitis and caries models, respectively.Conclusion In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia.