Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF).
Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF).
复制标题
DOI：
10.1186/s40729-016-0052-4
复制
发表时间：
2016-12
影响因子：
2.7
通讯作者：
Kawase T
中科院分区：
文献类型：
--
作者：
Masuki H;Okudera T;Watanebe T;Suzuki M;Nishiyama K;Okudera H;Nakata K;Uematsu K;Su CY;Kawase T
富血小板纤维蛋白（platelet-rich fibrin，PRF）的出现极大地简化了富血小板血浆（platelet-rich plasma，PRP）等血小板浓缩生物材料的制备过程，促进了其临床应用。PRF的临床有效性经常在临床前和临床研究中得到证实;然而，在PRF制剂中是否显著浓缩生长因子以促进伤口愈合和组织再生仍然存在争议。为了解决这个问题，我们进行了PRP及其衍生物，如先进的PRF（A-PRF）和浓缩生长因子（CGF）的生长因子含量的比较研究。PRP及其衍生物由从健康供体采集的相同外周血样品制备。将A-PRF和CGF制备物均质化并离心以产生提取物。A-PRF和CGF制剂中的血小板和白色血细胞计数通过从全血样品的计数中减去红细胞部分、上清液无细胞血清部分和A-PRF/CGF渗出物部分中的那些计数来确定。采用ELISA试剂盒测定生长因子（TGF-β1、PDGF-BB、VEGF）和促炎细胞因子（IL-1β、IL-6）的浓度。与PRP制剂相比，A-PRF和CGF提取物均含有相容或更高水平的血小板和血小板衍生生长因子。在细胞增殖测定中，A-PRF和CGF提取物均显著刺激人骨膜细胞的增殖，而在较高剂量下没有显著降低。这些数据清楚地表明，A-PRF和CGF制剂都含有大量能够刺激骨膜细胞增殖的生长因子，表明A-PRF和CGF制剂不仅作为支架材料，而且作为在应用部位递送某些生长因子的储存器。
The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF’s clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF). PRP and its derivatives were prepared from the same peripheral blood samples collected from healthy donors. A-PRF and CGF preparations were homogenized and centrifuged to produce extracts. Platelet and white blood cell counts in A-PRF and CGF preparations were determined by subtracting those counts in red blood cell fractions, supernatant acellular serum fractions, and A-PRF/CGF exudate fractions from those counts of whole blood samples. Concentrations of growth factors (TGF-β1, PDGF-BB, VEGF) and pro-inflammatory cytokines (IL-1β, IL-6) were determined using ELISA kits. Compared to PRP preparations, both A-PRF and CGF extracts contained compatible or higher levels of platelets and platelet-derived growth factors. In a cell proliferation assay, both A-PRF and CGF extracts significantly stimulated the proliferation of human periosteal cells without significant reduction at higher doses. These data clearly demonstrate that both A-PRF and CGF preparations contain significant amounts of growth factors capable of stimulating periosteal cell proliferation, suggesting that A-PRF and CGF preparations function not only as a scaffolding material but also as a reservoir to deliver certain growth factors at the site of application.