Drug-loaded nano/microbubbles for combining ultrasonography and targeted chemotherapy
Drug-loaded nano/microbubbles for combining ultrasonography and targeted chemotherapy
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DOI：
10.1016/j.ultras.2007.11.002
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发表时间：
2008-08-01
期刊：
影响因子：
4.2
通讯作者：
Rapoport, Natalya Y.
中科院分区：
文献类型：
--
作者：
Gao, Zhonggao;Kennedy, Anne M.;Rapoport, Natalya Y.
已经开发了一类新的多功能纳米颗粒，其结合了聚合物药物载体、超声成像造影剂和超声介导的药物递送的增强剂的联合收割机性质。在室温下，开发的系统包括由可生物降解的嵌段共聚物制成的壁稳定的全氟化碳纳米液滴。在加热到生理温度时，纳米液滴转化为纳米/微泡。体系的相态和气泡尺寸可以通过共聚物/全氟化碳体积比来控制。静脉注射后，在肿瘤体积中观察到持久、强烈和选择性的超声对比，表明纳米气泡通过缺陷肿瘤微血管外渗，表明它们在肿瘤组织中聚结成较大的高回声微气泡。在肿瘤导向超声的作用下，微泡空化和塌陷，导致包封的药物释放，并显著增强肿瘤细胞对细胞内药物的摄取。这种效应是肿瘤选择性的;在其他器官中未观察到回声微泡的积聚。使用该技术已经实现了MDA MB 231乳腺癌肿瘤的有效化疗。(C)2007 Elsevier B. V.保留所有权利。
A new class of multifunctional nanoparticles that combine properties of polymeric drug carriers, ultrasound imaging contrast agents, and enhancers of ultrasound-mediated drug delivery has been developed. At room temperature, the developed systems comprise perfluorocarbon nanodroplets stabilized by the walls made of biodegradable block copolymers. Upon heating to physiological temperatures, the nanodroplets convert into nano/microbubbles. The phase state of the systems and bubble size may be controlled by the copolymer/perfluorocarbon volume ratio. Upon intravenous injections, a long-lasting, strong and selective ultrasound contrast is observed in the tumor volume indicating nanobubble extravasation through the defective tumor microvasculature, suggesting their coalescence into larger, highly echogenic microbubbles in the tumor tissue. Under the action of tumor-directed ultrasound, microbubbles cavitate and collapse resulting in a release of the encapsulated drug and dramatically enhanced intracellular drug uptake by the tumor cells. This effect is tumor-selective; no accumulation of echogenic microbubbles is observed in other organs. Effective chemotherapy of the MDA MB231 breast cancer tumors has been achieved using this technique. (C) 2007 Elsevier B.V. All rights reserved.