A conformation- and ion-sensitive plasmonic biosensor.
A conformation- and ion-sensitive plasmonic biosensor.
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DOI：
10.1021/nl103994w
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发表时间：
2011-03-09
期刊：
影响因子：
10.8
通讯作者：
Van Duyne RP
中科院分区：
文献类型：
--
作者：
Hall WP;Modica J;Anker J;Lin Y;Mrksich M;Van Duyne RP
The versatile optical and biological properties of a localized surface plasmon resonance (LSPR) sensor that responds to protein conformational changes are illustrated. The sensor detects conformational changes in a surface-bound construct of the calcium-sensitive protein calmodulin. Increases in calcium concentration induce a 0.96 nm red-shift in the spectral position of the LSPR extinction maximum (λmax). Addition of a calcium chelating agent forces the protein to return to its original conformation and is detected as a reversal of the λmax shift. As opposed to previous work, this work demonstrates that these conformational changes produce a detectable shift in λmax even in the absence of a protein label, with a signal:noise ratio near 500. In addition, the protein conformational changes reversibly switch both the wavelength and intensity of the resonance peak, representing an example of a bimodal plasmonic component that simultaneously relays two distinct forms of optical information. This highly versatile plasmonic device acts as a biological sensor, enabling the detection of calcium ions with a biologically-relevant limit of detection of 23 μM, as well as the detection of calmodulin-specific protein ligands.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.072685299
发表时间：
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