Phospholipid Vesicles in Media for Tribological Studies against Live Cartilage.
Phospholipid Vesicles in Media for Tribological Studies against Live Cartilage.
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DOI：
10.3390/lubricants6010019
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发表时间：
2018-02
期刊：
影响因子：
3.5
通讯作者：
Teresa Veselack;Grégory Aldebert;A. Trunfio-Sfarghiu;T. Schmid;M. Laurent;M. Wimmer
中科院分区：
文献类型：
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作者：
Teresa Veselack;Grégory Aldebert;A. Trunfio-Sfarghiu;T. Schmid;M. Laurent;M. Wimmer
介绍半关节置换术设备的临床前测试要求与活体软骨的摩擦学条件紧密复制。目前活体软骨摩擦学测试的一个局限性是使用细胞培养液作为润滑剂。研究目的是开发和测试一种新型的透明质酸磷脂基质(HA磷脂介质)，该介质将滑液的流变性和摩擦性与培养液的营养特性相结合，以保持细胞的存活。材料与方法HA磷脂培养基组分为添加磷脂的二棕榈酰磷脂酰胆碱(0.3 mg/mL)和透明质酸(2.42 mg/mL)。以标准细胞培养基作对照。使用平板构型测定每种介质的流变性。用牛小腿软骨细胞在每种培养液中测定细胞活力和摩擦力。为了测量摩擦力，将钴铬合金球关节连接到浸泡在介质中的软骨盘上。结果在HA-磷脂基质中可观察到直径为0.1~50μm的脂泡。软骨细胞在HA-磷脂培养液中的存活率(62%±8%，95%CI)显著高于对照组(49.5%±5%)(p=0.009)。HA磷脂基质表现出强烈的剪切稀化行为，与滑膜液相似，在10 S-1和20,000 S-1下的粘度分别是牛顿对照介质的100倍和5倍。经过1小时的测试，HA磷脂介质的摩擦值也比对照介质低20%。结论流变学和摩擦学实验结果表明，HA磷脂培养基比对照细胞培养基能更好地模拟滑液的剪切稀释性和润滑性，更适合于活体软骨的体外磨损和摩擦试验。
Introduction Pre-clinical testing of hemiarthroplasty devices requires that the tribological conditions present in vivo with live cartilage be closely duplicated. A current limitation in the tribological testing of live cartilage involves the use of cell-culture media as lubricant. Study Aim to develop and test a new hyaluronan-phospholipid based medium (HA-phospholipid medium) that combines the rheological and frictional properties of synovial fluid with the nourishing properties of culture media to keep cells alive. Materials and Methods The HA-phospholipid medium consisted of culture medium with added phospholipid dipalmitoylphosphatidylcholine (0.3 mg/mL), and hyaluronic acid (2.42 mg/mL). A standard cell culture medium was used as the control. The rheology of each medium was determined using a flat plate configuration. Bovine calf cartilage was used to assess cell viability and friction in each medium. For friction measurements, a cobalt-chrome alloy ball was articulated against cartilage disks immersed in medium. Results Lipid vesicles 0.1 to 50 μm in diameter were identified in the HA-phospholipid medium. Cartilage cell viability was significantly higher in the HA-phospholipid medium (62% ± 8%, 95% CI) than in control medium (49.5% ± 5%) (p = 0.009). The HA-phospholipid medium exhibited strong shear-thinning behavior, similar to synovial fluid, with viscosities ~100-fold higher at 10 s-1 and 5-fold higher at 20,000 s-1 than the approximately Newtonian control medium. The HA-phospholipid medium also yielded 20% lower friction values than the control medium after one hour of testing. Conclusions The rheological and friction results indicate that the HA-phospholipid medium is superior to the control cell culture medium in emulating the shear thinning and lubricative properties of natural synovial fluid, making it more clinically relevant for in vitro wear and friction testing with live cartilage.