Kinome Expansion in the Fusarium oxysporum Species Complex Driven by Accessory Chromosomes.
Kinome Expansion in the Fusarium oxysporum Species Complex Driven by Accessory Chromosomes.
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DOI：
10.1128/msphere.00231-18
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发表时间：
2018-06-27
期刊：
影响因子：
4.8
通讯作者：
Ma LJ
中科院分区：
文献类型：
--
作者：
DeIulio GA;Guo L;Zhang Y;Goldberg JM;Kistler HC;Ma LJ
尖孢镰刀菌的分离物适应于在广泛的宿主和非宿主条件下存活。此外，尖孢镰刀菌最近被认为是感染免疫功能低下的人类的顶级新兴机会性真菌病原体。这些真菌的感觉和反应网络无疑在建立这一群体的适应性方面发挥了重要作用。我们已经检查了12个尖孢镰刀菌分离株的激酶组，并强调了区分尖孢镰刀菌与其他真菌以及不同分离株的激酶家族。参与环境信号传递和调节下游细胞反应的激酶的扩增清楚地将镰刀菌与其他子囊菌区分开来。虽然这些激酶中的许多的功能仍然不清楚，但它们的特定增殖突出了它们作为形成这种物种复合体的进化力量的结果，并清楚地将它们标记为利用的目标，以对抗疾病。尖孢镰刀菌复合体（Fusarium oxysporum species complex，FOSC）是一组土传病原菌，可在100多种植物宿主中引起严重病害，而单个菌株表现出很强的宿主特异性。染色体转移和比较基因组学实验都表明，谱系特异性（LS）染色体有助于宿主特异性致病性。然而，很少有人知道这些LS染色体中编码的基因的功能的重要性。该研究重点关注信号转导，比较了12个尖孢镰刀菌分离株的激酶组，包括植物和人类病原体以及1个非致病性生物控制菌株，以及7个额外的公开的子囊菌基因组。总的来说，尖孢镰刀菌的激酶组是最大的，部分是由于LS染色体的获得而促进的。这项比较研究确定了几乎所有检测的真菌基因组中存在的99种激酶，形成了子囊菌真菌的核心信号网络。与保守的子囊菌激酶组相比，尖孢镰刀菌激酶组的扩增发生在几个激酶家族中，例如参与环境信号传感的组氨酸激酶和介导细胞应答的雷帕霉素（TOR）激酶的靶标。比较激酶组分析表明，一个趋同的进化，形成个别尖孢镰刀菌菌株具有增强的和独特的能力，环境感知和相关的下游反应。重要提示尖孢镰刀菌的分离株能够适应各种宿主和非宿主条件。此外，尖孢镰刀菌最近被认为是感染免疫功能低下的人类的顶级新兴机会性真菌病原体。这些真菌的感觉和反应网络无疑在建立这一群体的适应性方面发挥了重要作用。我们已经检查了12个尖孢镰刀菌分离株的激酶组，并强调了区分尖孢镰刀菌与其他真菌以及不同分离株的激酶家族。参与环境信号传递和调节下游细胞反应的激酶的扩增清楚地将镰刀菌与其他子囊菌区分开来。虽然这些激酶中的许多的功能仍然不清楚，但它们的特定增殖突出了它们作为形成这种物种复合体的进化力量的结果，并清楚地将它们标记为利用的目标，以对抗疾病。
Isolates of Fusarium oxysporum are adapted to survive a wide range of host and nonhost conditions. In addition, F. oxysporum was recently recognized as the top emerging opportunistic fungal pathogen infecting immunocompromised humans. The sensory and response networks of these fungi undoubtedly play a fundamental role in establishing the adaptability of this group. We have examined the kinomes of 12 F. oxysporum isolates and highlighted kinase families that distinguish F. oxysporum from other fungi, as well as different isolates from one another. The amplification of kinases involved in environmental signal relay and regulating downstream cellular responses clearly sets Fusarium apart from other Ascomycetes. Although the functions of many of these kinases are still unclear, their specific proliferation highlights them as a result of the evolutionary forces that have shaped this species complex and clearly marks them as targets for exploitation in order to combat disease. The Fusarium oxysporum species complex (FOSC) is a group of soilborne pathogens causing severe disease in more than 100 plant hosts, while individual strains exhibit strong host specificity. Both chromosome transfer and comparative genomics experiments have demonstrated that lineage-specific (LS) chromosomes contribute to the host-specific pathogenicity. However, little is known about the functional importance of genes encoded in these LS chromosomes. Focusing on signaling transduction, this study compared the kinomes of 12 F. oxysporum isolates, including both plant and human pathogens and 1 nonpathogenic biocontrol strain, with 7 additional publicly available ascomycete genomes. Overall, F. oxysporum kinomes are the largest, facilitated in part by the acquisitions of the LS chromosomes. The comparative study identified 99 kinases that are present in almost all examined fungal genomes, forming the core signaling network of ascomycete fungi. Compared to the conserved ascomycete kinome, the expansion of the F. oxysporum kinome occurs in several kinase families such as histidine kinases that are involved in environmental signal sensing and target of rapamycin (TOR) kinase that mediates cellular responses. Comparative kinome analysis suggests a convergent evolution that shapes individual F. oxysporum isolates with an enhanced and unique capacity for environmental perception and associated downstream responses. IMPORTANCE Isolates of Fusarium oxysporum are adapted to survive a wide range of host and nonhost conditions. In addition, F. oxysporum was recently recognized as the top emerging opportunistic fungal pathogen infecting immunocompromised humans. The sensory and response networks of these fungi undoubtedly play a fundamental role in establishing the adaptability of this group. We have examined the kinomes of 12 F. oxysporum isolates and highlighted kinase families that distinguish F. oxysporum from other fungi, as well as different isolates from one another. The amplification of kinases involved in environmental signal relay and regulating downstream cellular responses clearly sets Fusarium apart from other Ascomycetes. Although the functions of many of these kinases are still unclear, their specific proliferation highlights them as a result of the evolutionary forces that have shaped this species complex and clearly marks them as targets for exploitation in order to combat disease.