Characterization of Escherichia coli cells deficient in 1-acyl-sn-glycerol-3- phosphate acyltransferase activity.
Characterization of Escherichia coli cells deficient in 1-acyl-sn-glycerol-3- phosphate acyltransferase activity.
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DOI：
10.1016/s0021-9258(17)44891-5
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发表时间：
1990-10
期刊：
影响因子：
--
通讯作者：
Jack Coleman
中科院分区：
文献类型：
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作者：
Jack Coleman
大肠杆菌K-12的1-酰基-sn-甘油-3-磷酸酰基转移酶缺陷突变株已被分离。在允许生长的温度30 ℃下，总细胞甘油磷脂的20%是1-酰基-sn-甘油-3-磷酸，如通过质谱分析和质子NMR所鉴定的。当培养物的温度变为42 ℃时，1-酰基-sn-甘油-3-磷酸的百分比上升至约30%。这种增加主要是以磷脂酰乙醇胺为代价的。来自携带plsC突变的细胞的提取物没有可检测的1-酰基-sn-甘油-3-磷酸酰基转移酶活性。累积的1-酰基-sn-甘油-3-磷酸的脂肪酸组成为约60%顺式-异油酸酯和40%棕榈酸酯，没有可检测量的棕榈油酸酯或其他脂肪酸，与甘油磷脂的sn-1位的已知脂肪酸组成一致。plsC基因的分离完成了已知在大肠杆菌中合成主要甘油磷脂所需的基因列表。杆菌
A mutant of Escherichia coli K-12 defective in 1-acyl-sn-glycerol-3-phosphate acyltransferase has been isolated. At the permissive temperature for growth, 30 degrees C, 20% of the total cellular glycerophospholipids is 1-acyl-sn-glycerol-3-phosphate, as identified by mass spectral analysis and proton NMR. This percentage of 1-acyl-sn-glycerol-3-phosphate rises to about 30% when the temperature of the culture is shifted to 42 degrees C. This increase is primarily at the expense of phosphatidylethanolamine. Extracts from cells harboring the plsC mutation have no detectable 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. The fatty acid composition of the accumulated 1-acyl-sn-glycerol-3-phosphate is about 60% cis-vaccenate and 40% palmitate, with no detectable amounts of palmitoleate or other fatty acids, consistent with the known fatty acid composition of the sn-1 position of glycerophospholipids. The isolation of this gene, plsC, completes the list of genes known to be required for the synthesis of the major glycerophospholipids in E. coli.