The usefulness of fatty acid analysis in differentiating species of Pythium, Rhizoctonia and Gaeumannomyces and as a tool for their detection in infected wheat roots
The usefulness of fatty acid analysis in differentiating species of Pythium, Rhizoctonia and Gaeumannomyces and as a tool for their detection in infected wheat roots
复制标题
脂肪酸分析在区分腐霉属、丝核菌属和全能酵母属物种以及作为检测受感染小麦根部的工具中的有用性
DOI：
10.1071/ap01018
复制
发表时间：
2001
影响因子：
1.4
通讯作者：
B. Hawke
中科院分区：
文献类型：
--
作者：
C. Pankhurst;H. Pederson;B. Hawke
从盖氏酵母、立枯丝核菌和腐霉培养物中提取的细胞脂肪酸的主成分分析。显示出属内文化的强烈聚集性和属间良好的区分性。脂肪酸图谱的主成分分析成功地区分了7种不同的腐霉。试验(P.arrhenomannes、P.australe、P.echinulatum、P.irRegare、P.ostraco des、P.sp.spinosum和P.ultimum)，但不能区分Gaeumannmyces spp.(G.graminis var.)Avenae、禾谷类(G.graminis var.)Graminis、G.graminis var.Tritici、柱状孢子菌和结瘤丝核菌)，也没有检测到丝核菌的两个AG组的分离物。不同物种间各脂肪酸的相对含量存在显著差异。两种脂肪酸(20：4ω6c和20：5ω3c)的存在被用来检测被这两种真菌侵染的小麦根中是否存在棘突疫霉和不规则疫霉。
Principal component analysis (PCA) of cellular fatty acids extracted from cultures of Gaeumannomyces spp., Rhizoctonia solani (anastamosis groups AG4 and AG8) and Pythium spp. showed strong clustering of cultures within genera and good discrimination between genera. PCA of the fatty acid profiles was successful in distinguishing between seven different Pythium spp. tested (P. arrhenomannes, P. australe, P. echinulatum, P. irregulare, P. ostracodes, P. spinosum and P. ultimum) but did not distinguish between the Gaeumannomyces spp. (G. graminis var. avenae, G. graminis var. graminis, G. graminis var. tritici, G. cylindrosporus and G. incrustans) nor the isolates of the two AG groups of Rhizoctonia examined. Significant differences between species were detected in the relative amounts of individual fatty acids. The presence of two fatty acids (20:4ω6c and 20:5ω3c), found only in the Pythium species examined, was used to detect the presence of P. echinulatum and P. irregulare in wheat roots infected with these two fungi.