Global profiling of phytohormone dynamics during combined drought and pathogen stress in Arabidopsis thaliana reveals ABA and JA as major regulators.
Global profiling of phytohormone dynamics during combined drought and pathogen stress in Arabidopsis thaliana reveals ABA and JA as major regulators.
复制标题
DOI：
10.1038/s41598-017-03907-2
复制
发表时间：
2017-06-21
影响因子：
4.6
通讯作者：
Senthil-Kumar M
中科院分区：
文献类型：
--
作者：
Gupta A;Hisano H;Hojo Y;Matsuura T;Ikeda Y;Mori IC;Senthil-Kumar M
全球转录组研究表明，存在独特的植物反应，在联合应力，否则不会看到在单独的压力。为了对抗复合胁迫，植物使用信号通路和由参与胁迫和生长相关过程的激素介导的“串扰”。然而，在复合胁迫植物中激素途径之间的相互作用尚不清楚。本文采用液相色谱-质谱联用技术研究了干旱和病原菌单独及联合侵染下拟南芥不同激素的动态变化。结果表明，脱落酸（阿坝）和水杨酸（SA）分别是干旱和病原菌胁迫下的关键调节因子。在干旱和寄主病原体胁迫（DH）相结合的情况下，我们观察到非诱导水平的阿坝与SA和茉莉酸（JA）浓度的激增，强调它们在基础耐受寄主病原体的作用。在非寄主病原菌与干旱胁迫（DNH）互作下，阿坝、SA和JA的含量变化与DH或非寄主病原菌单独作用下的相似。我们认为，植物在DH胁迫过程中使用SA/JA依赖的信号传导，在胁迫早期拮抗阿坝的生物合成和信号传导途径。这项研究提供了在复合应激期间不同时间点激素调节的见解。
Global transcriptome studies demonstrated the existence of unique plant responses under combined stress which are otherwise not seen during individual stresses. In order to combat combined stress plants use signaling pathways and ‘cross talk’ mediated by hormones involved in stress and growth related processes. However, interactions among hormones’ pathways in combined stressed plants are not yet known. Here we studied dynamics of different hormones under individual and combined drought and pathogen infection in Arabidopsis thaliana by liquid chromatography-mass spectrometry (LC-MS) based profiling. Our results revealed abscisic acid (ABA) and salicylic acid (SA) as key regulators under individual drought and pathogen stress respectively. Under combined drought and host pathogen stress (DH) we observed non-induced levels of ABA with an upsurge in SA and jasmonic acid (JA) concentrations, underscoring their role in basal tolerance against host pathogen. Under a non-host pathogen interaction with drought (DNH) stressed plants, ABA, SA and JA profiles were similar to those under DH or non-host pathogen alone. We propose that plants use SA/JA dependent signaling during DH stress which antagonize ABA biosynthesis and signaling pathways during early stage of stress. The study provides insights into hormone modulation at different time points during combined stress.