白菜中过表达miR158导致花粉败育
白菜中过表达miR158导致花粉败育
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
曹家树
中科院分区：
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作者：
马智明;JianxiaJiang;ZiweiHu;TianqiLyu;YangYang;JingjingJiang;曹家树
MicroRNAs （miRNAs）通过mRNA切割或翻译抑制调控靶基因的表达，在植物和动物的许多重要生长发育过程中起着至关重要的作用。本研究对芸苔科特异miRNA miR158在无头大白菜花粉发育中的作用进行了功能鉴定。对)。鉴定了油菜分枝杆菌miR158的两个家族成员bra-miR158a1和bra-miR158a2及其靶基因bra027656，该基因编码一种含PPR的蛋白。qRT-PCR分析和gus报告系统分析显示，bra-miR158及其靶基因在花序中均有较高的表达水平。进一步研究发现，miR158过表达导致花粉变异性和花粉萌发率降低，畸形花粉粒中双核小孢子期花粉含量下降，导致花粉发育后期花粉萎缩和崩落。这些结果首次揭示了miR158在油菜花粉发育中的重要性。对。
MicroRNAs (miRNAs) play crucial roles in many important growth and development processes both in plants and animals by regulating the expression of their target genes via mRNA cleavage or translational repression. In this study, miR158, a Brassicaceae specific miRNA, was functionally characterized with regard to its role in pollen development of non-heading Chinese cabbage (Brassica campestris ssp. chinensis). Two family members of miR158 in B. campestris, namely bra-miR158a1 and bra-miR158a2, and their target gene bra027656, which encodes a pentatricopeptide repeat (PPR) containing protein, were identified. Then, qRT-PCR analysis and GUS-reporter system revealed that both bra-miR158 and its target gene had relatively high expression levels in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility and pollen germination ratio, and the degradation of pollen contents from the binucleate microspore stage was also found in those deformed pollen grains, which led to pollen shrinking and collapse in later pollen development stage. These results first shed light on the importance of miR158 in pollen development of Brassica campestris ssp. chinensis.