Application of immunoaffinity chromatography for detection of tetrodotoxin from urine samples of poisoned patients
Application of immunoaffinity chromatography for detection of tetrodotoxin from urine samples of poisoned patients
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DOI：
10.1016/s0041-0101(98)00116-0
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发表时间：
1999-02-01
期刊：
影响因子：
2.8
通讯作者：
Hamano, E
中科院分区：
文献类型：
--
作者：
Kawatsu, K;Shibata, T;Hamano, E
建立了河豚毒素（TTX）单克隆抗体免疫亲和层析法。将免疫亲和层析与荧光高效液相层析（HPLC）相结合，可以检测尿样中的微量TTX。尿中TTX的检出限为2ng/ml。通过这种联合方法，TTX中毒患者在摄入TTX后一周内收集的所有尿液样品中均检测到TTX。免疫亲和层析与高效液相色谱联用检测中毒患者尿液中的TTX，对诊断TTX食物中毒有重要意义。(C)1999年爱思唯尔科学有限公司。保留所有灯光。
Immunoaffinity chromatography using the monoclonal antibody (TI-I) specific for tetrodotoxin (TTX) has been developed for isolating TTX from urine samples. By combining immunoaffinity chromatography with fluorometric high performance liquid chromatography (HPLC), it has become possible to detect a small amount of TTX in urine samples. The detection limit of TTX in urine was 2 ng/ml. By this combined method, TTX was detected in all the urine samples that were collected from poisoned patients during the week following TTX ingestion. The combination of immunoaffinity chromatography with HPLC was very useful in detecting TTX from the urine samples of poisoned patients for diagnosis of TTX-food poisoning. (C) 1999 Elsevier Science Ltd. All lights reserved.