Endonuclease G: A role for the enzyme in recombination and cellular proliferation
Endonuclease G: A role for the enzyme in recombination and cellular proliferation
复制标题
DOI：
10.1073/pnas.0603445103
复制
发表时间：
2006-06-13
影响因子：
11.1
通讯作者：
Lehman, I. Robert
中科院分区：
文献类型：
--
作者：
Huang, Ke-Jung;Ku, Chia-Chi;Lehman, I. Robert
我们早期的研究表明，内切核酸酶G（EndoG），进化上保守的DNA/RNA非特异性ss α-Me-指核酸酶家族的成员，在单纯疱疹病毒1型复制过程中观察到的序列介导的片段倒位中发挥作用。为了验证这一假设，我们使用RNA干扰来降低培养的哺乳动物细胞中EndoG的水平。EndoG的减少在序列介导的重组中产生了小的但统计学上显著的减少，表明EndoG在该过程中确实起作用。我们还观察到EndoG水平的降低导致细胞增殖的缺陷。EndoG水平降低的细胞也显示出细胞周期中细胞分布的变化，产生了在G（2）期停滞的细胞的特征性模式。这些发现表明，EndoG是正常细胞增殖所必需的。
Our earlier studies had suggested that endonuclease G (EndoG), a member of the evolutionarily conserved DNA/RNA nonspecific ss alpha-Me-finger nuclease family, functioned in the a sequence-mediated segment inversion observed during herpes simplex virus 1 replication. To test this hypothesis, we used RNA interference to reduce the level of EndoG in mammalian cells in culture. Reduction of EndoG produced a small but statistically significant decrease in a sequence-mediated recombination, suggesting that EndoG does play a role in this process. We also observed that reduction in the level of EndoG resulted in a deficiency in cell proliferation. Cells with a reduced level of EndoG also showed changes in cell distribution in the cell cycle, producing a pattern characteristic of cells that have been arrested in the G(2) phase. These findings suggest that EndoG is required for normal cellular proliferation.