Substrate specificity determinants of the checkpoint protein kinase Chk1
Substrate specificity determinants of the checkpoint protein kinase Chk1
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DOI：
10.1016/s0014-5793(99)01763-9
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发表时间：
2000-01-21
期刊：
影响因子：
3.5
通讯作者：
Clarke, PR
中科院分区：
文献类型：
--
作者：
Hutchins, JRA;Hughes, M;Clarke, PR
Chk 1蛋白激酶在裂殖酵母和动物之间保守的DNA损伤检查点途径中起关键作用。我们已经开发了一种Chk 1活性的定量测定，使用来自含有Ser-287（Chk 1的已知靶标）的非洲爪蟾Cdc 25 C区域的肽，该肽的变体用于确定参与Chk 1底物识别的残基，揭示磷酸化基序Phi-X-β-X-X-（S/T）*，其中 * 表示磷酸化残基，Phi是疏水残基（M > I > L > V），β是碱性残基（R > K），X是任何氨基酸。该基序表明Chk 1是一组应激反应蛋白激酶的成员，其磷酸化具有相关特异性的靶蛋白。(C)2000年欧洲生物化学学会联合会。
The Chk1 protein kinase plays a critical role in a DNA damage checkpoint pathway conserved between fission yeast and animals. We have developed a quantitative assay for Chk1 activity, using a peptide derived from a region of Xenopus Cdc25C containing Ser-287, a known target of Chk1, Variants of this peptide were used to determine the residues involved in substrate recognition by Chk1, revealing the phosphorylation motif Phi-X-beta-X-X-(S/T)*, where * indicates the phosphorylated residue, Phi is a hydrophobic residue (M > I > L > V), beta is a basic residue (R > K) and X is any amino acid. This motif suggests that Chk1 is a member of a group of stress-response protein kinases which phosphorylate target proteins with related specificities. (C) 2000 Federation of European Biochemical Societies.