Effect of sporulation and recovery medium on the heat resistance and amount of injury of spores from spoilage bacilli
Effect of sporulation and recovery medium on the heat resistance and amount of injury of spores from spoilage bacilli
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DOI：
10.1046/j.1365-2672.2001.01302.x
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发表时间：
2001-05-01
影响因子：
4
通讯作者：
ter Steeg, PF
中科院分区：
文献类型：
--
作者：
Cazemier, AE;Wagenaars, SFM;ter Steeg, PF
目的：评价产孢介质对耐热性的影响，并利用应激恢复介质测定5种具有代表性的腐败杆菌孢子的保存损伤。方法与结果：芽孢杆菌孢子在添加Ca2+、Mg2+、Mn2+、Fe2+和K+的营养琼脂上的耐热性优于添加Mn2+的营养琼脂。这种增加的耐热性与原生质体含水量的减少有关，这是由浮力密度沉积决定的。在含有中等pH的NaCl和酸性有机酸的培养基中，可以评估保存损伤的严重程度。在恢复培养基中添加Ca-DPA、K+或脯氨酸，表明热可能对孢子萌发和生长系统都造成了伤害。研究的意义和影响：产孢培养基的金属含量对保存抗性研究的有效性有很大影响。这里开发的独特的恢复介质可以用于评估和比较新的保存技术。
Aims: To assess the influence of sporulation media on heat resistance, and the use of stress recovery media to measure preservation injury of spores of five representative spoilage bacilli.Methods and Results: Bacillus spores prepared on nutrient agar supplemented with Ca2+, Mg2+, Mn2+, Fe2+ and K+ were more heat-resistant than spores obtained from nutrient agar with Mn2+. This increased heat resistance correlated with a decrease in the protoplast water content as determined by buoyant density sedimentation. The degree of preservation injury severity could be assessed on media containing NaCl at moderate pH and organic acids at acid pH. Ca-DPA, K+ or proline were added to the recovery media to demonstrate that heat probably caused injury to both spore germination and the outgrowth system.Significance and Impact of the Study: The metal content of sporulation media can strongly effect the validity of preservation resistance studies. The distinctive recovery media developed here can be relevant for assessing and comparing new preservation technologies.