INFLUENZA VIRUS-M2 PROTEIN IS AN INTEGRAL MEMBRANE-PROTEIN EXPRESSED ON THE INFECTED-CELL SURFACE
INFLUENZA VIRUS-M2 PROTEIN IS AN INTEGRAL MEMBRANE-PROTEIN EXPRESSED ON THE INFECTED-CELL SURFACE
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DOI：
10.1016/0092-8674(85)90211-9
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
RICHARDSON, CD
中科院分区：
文献类型：
--
作者：
LAMB, RA;ZEBEDEE, SL;RICHARDSON, CD
甲型流感病毒M2蛋白在细胞表面大量表达，是除血凝素（HA）和神经氨酸酶（NA）之外的第3种病毒特异性膜蛋白。M2具有内部疏水膜锚定结构域，并与HA和NA相同的细胞膜组分结合。胰蛋白酶处理感染的CV-1细胞和免疫沉淀与位点特异性抗血清表明，至少有18个NH 2-末端氨基酸的M2暴露在细胞表面。10个NH 2-末端残基在可获得序列的所有甲型流感病毒株中是保守的。抗体可以识别细胞表面上的M2，因此它可能是感染的细胞表面抗原。M2的特性，它匹配的难以捉摸的主要靶分子对流感病毒A感染的细胞交叉反应性细胞毒性T细胞进行了讨论。
The influenza A virus M2 protein is expressed abundantly at the cell surface, and in addition to the hemagglutinin (HA) and neuraminidase (NA), is a 3rd virus-specific membrane protein. M2 has an internal hydrophobic membrane anchorage domain and associates with the same cellular membrane fractions as HA and NA. Trypsin treatment of infected CV-1 cells and immunoprecipitation with site-specific antisera indicate that a minimum of 18 NH2-terminal amino acids of M2 are exposed at the cell surface. Ten NH2-terminal residues are conserved in all strains of influenza A virus for which sequences are available. Antibodies can recognize M2 on the cell surface and therefore it may be an infected-cell surface antigen. Properties of M2 that match it to the elusive major target molecule on influenza A virus-infected cells for crossreactive cytotoxic T cells are discussed.