A novel biogenic Allium cepa leaf mediated silver nanoparticles for antimicrobial, antioxidant, and anticancer effects on MCF-7 cell line
A novel biogenic Allium cepa leaf mediated silver nanoparticles for antimicrobial, antioxidant, and anticancer effects on MCF-7 cell line
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DOI：
10.1016/j.envres.2021.111199
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发表时间：
2021-05-04
影响因子：
8.3
通讯作者：
Elshikh, Mohamed Soliman
中科院分区：
文献类型：
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作者：
Mani, M.;Okla, Mohammad K.;Elshikh, Mohamed Soliman
在本研究中，利用洋葱叶提取物将硝酸银还原成纳米级银离子（Ag NP）。通过 X 射线衍射分析 (XRD)、动态光散射分析 (DLS)、紫外-可见光谱 (UV-vis)、透射电子显微镜 (TEM)、能量色散 X 射线分析 (EDX) 和傅里叶变换红外光谱 (FTIR) 对生物合成的银纳米颗粒进行了广泛的表征。合成的Ag NPs 的抗氧化活性通过DPPH 测定进行验证。根据 XRD 和 DLS 研究的结果，Ag 纳米颗粒的尺寸确定为 54.3 nm 左右。测得的 zeta 电位值为 -19.1 mV，证实了生物合成的 Ag NPs 具有优异的稳定性。 TEM 分析表明生物合成的 Ag NPs 具有尺寸为 13 nm 的球形结构。通过 FTIR 研究证实了各种官能团的存在，EDAX 验证了生物合成纳米颗粒中银含量的重量百分比为 30.33%。在本研究中，利用乳腺癌细胞系MCF-7进行抗癌活性。此外，银纳米粒子对革兰氏阳性蜡样芽孢杆菌和革兰氏阴性大肠杆菌表现出抗菌功效。 MTT 测定还显示出针对 MCF-7 细胞系的更好的细胞毒活性。
In the present study, Allium cepa leaf extract was utilized to reduce the silver nitrate into the nanoscale range of silver ions (Ag NPs). The biosynthesized Ag NPs were extensively characterized by X-ray diffraction analysis (XRD), Dynamic light scattering analysis (DLS), UV-Visible spectroscopy (UV-vis), Transmission electron microscopy (TEM), Energy dispersive X-ray analysis (EDX) and Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of synthesized Ag NPs was verified by DPPH assay. From the results obtained from XRD and DLS studies, the size of Ag NPs was determined to be around 54.3 nm. The measured zeta potential value of -19.1 mV confirms the excellent stability of biosynthesized Ag NPs. TEM analyses reveal that the biosynthesized Ag NPs have a spherical structure of 13 nm in size. The presence of various functional groups was confirmed through FTIR studies and EDAX verifies the weight percentage of silver content in biosynthesized nanoparticles to be 30.33%. In the present study, anti-cancer activity was carried out by using breast cancer cell line MCF-7. Further, silver nanoparticles exhibited antimicrobial effectiveness against gram-positive Bacillus cereus and gramnegative Escherichia coli. The MTT assay also showed better cytotoxic activity against the MCF- 7 cell line.