Efficient use of a 'dead-end' GA 5′ splice site in the human fibroblast growth factor receptor genes
Efficient use of a 'dead-end' GA 5′ splice site in the human fibroblast growth factor receptor genes
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DOI：
10.1093/emboj/cdg163
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发表时间：
2003-04-01
期刊：
影响因子：
11.4
通讯作者：
Screaton, GR
中科院分区：
文献类型：
--
作者：
Brackenridge, S;Wilkie, AOM;Screaton, GR
我们研究了脊椎动物成纤维细胞生长因子受体（FGFR）基因中保守的非经典GA 5'剪接位点的使用。尽管以前的研究表明，GA在内含子的开始是不兼容的剪接，我们观察到有效利用这个剪接位点的人FGFR1基因构建体。我们表明，GA剪接位点的使用依赖于上游6个核苷酸的常规剪接位点和下游内含子内的序列元件。此外，我们的结果与串联5'剪接位点之间的竞争是由U6 snRNP介导的，而不是U1 snRNP。因此，GA 5 ′剪接位点代表相邻常规5 ′剪接位点的延伸，这是这种复合5 ′剪接位点的第一个天然实例。
We have investigated use of a conserved non-canonical GA 5' splice site present in vertebrate fibroblast growth factor receptor ( FGFR) genes. Despite previous studies suggesting that GA at the beginning of an intron is incompatible with splicing, we observe efficient utilization of this splice site for human FGFR1 gene constructs. We show that use of the GA splice site is dependent on both a conventional splice site six nucleotides upstream and sequence elements within the downstream intron. Furthermore, our results are consistent with competition between the tandem 5' splice sites being mediated by U6 snRNP, rather than U1 snRNP. Thus the GA 5' splice site represents an extension of the adjacent conventional 5' splice site, the first natural example of such a composite 5' splice site.