Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples.
Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples.
复制标题
通过盲肠和粪便样本评估肠道微生物群对猪脂肪变化的贡献
DOI：
10.3389/fmicb.2016.02108
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发表时间：
2016
影响因子：
5.2
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
He M;Fang S;Huang X;Zhao Y;Ke S;Yang H;Li Z;Gao J;Chen C;Huang L
胃肠道微生物群落参与了人体肥胖及多种代谢性疾病的发生发展。然而，关于肠道微生物区系与肥育猪之间关系的研究很少。在这里，我们使用高通量测序技术对二花脸猪256份盲肠和八马乡猪244份粪便样品进行了16S rRNA基因分析，并采用两部分模型统计方法评估肠道微生物与猪肥胖度的相关性。作为结果，我们在粪便和盲肠样本中分别鉴定了6个和108个操作分类单位(OTU)，以及9个和10个与脂肪性状显著相关的细菌分类群。交叉验证分析表明，肠道微生物组对腹脂含量的影响最大，可解释腹脂重2.73%的表型变异。盲肠样本中与肥胖相关的OTUS明显多于大便样本，这表明盲肠腔样本比大便更能用于肥胖相关微生物的鉴定。与肥胖相关的OTUS主要注释为乳螺科、反刍球菌科、普雷沃特氏菌、密螺旋体和类杆菌。据报道，这些微生物通过发酵饮食中未消化的多糖和果胶来产生短链脂肪酸。短链脂肪酸可以调节宿主体内能量平衡，保护宿主免受炎症反应，抑制脂肪团的形成。我们的发现表明，肠道微生物群可能是调节猪肥胖的一个重要因素。
Microbial community in gastrointestinal tract participates in the development of the obesity as well as quite a few metabolic diseases in human. However, there are few studies about the relationship between gut microbiota and porcine fatness. Here, we used high-throughput sequencing to perform 16S rRNA gene analysis in 256 cecum luminal samples from Erhualian pigs and 244 stools from Bamaxiang pigs, and adopted a two-part model statistical method to evaluate the association of gut microbes with porcine fatness. As the results, we identified a total of 6 and 108 operational taxonomic units (OTUs), and 9 and 10 bacterial taxa which showed significant associations with fatness traits in the stool and cecum samples, respectively. Cross-validation analysis indicated that gut microbiome showed the largest effect on abdominal adipose by explaining 2.73% phenotypic variance of abdominal fat weight. Significantly more fatness-associated OTUs were identified in the cecum samples than that in the stools, suggesting that cecum luminal samples were better used for identification of fatness-associated microbes than stools. The fatness-associated OTUs were mainly annotated to Lachnospiraceae, Ruminococcaceae, Prevotella, Treponema, and Bacteroides. These microbes have been reported to produce short-chain fatty acids by fermenting dietary indigested polysaccharide and pectin. The short-chain fatty acids can regulate host body energy homeostasis, protect host from inflammation and inhibit fat mass development. Our findings suggested that the gut microbiome may be an important factor modulating fatness in pigs.