Toxin-resistant calcium currents, in embryonic mouse sensory neurons
Toxin-resistant calcium currents, in embryonic mouse sensory neurons
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DOI：
10.1016/s0306-4522(97)00101-2
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发表时间：
1997-09-01
期刊：
影响因子：
3.3
通讯作者：
Valmier, J
中科院分区：
文献类型：
--
作者：
Hilaire, C;Diochot, S;Valmier, J
We characterized toxin-insensitive calcium currents expressed by acutely dissociated embryonic dorsal root ganglion neurons. In the presence of 3 mu M omega-conotoxin-GVIA, 3 mu M nitrendipine and either 500 nM omega-agatoxin-IVA or 500 nM omega-conotoxin-MVIIC to inhibit N-, L- and P/Q-type currents, respectively, all neurons expressed two residual currents: a T-type and another which we referred to as toxin-resistant current. The toxin-resistant current (i) consisted of an inactivating and a sustained components, (ii) had a threshold of activation and a steady-state inactivation comprised between that of the T-type current and that of the other high-voltage-activated currents, (iii) had the same permeability for barium and calcium used as charge carriers, (iv) was highly sensitive to both cadmium and nickel; and (v) was insensitive to 500 mu M amiloride which abolished the T-type at this concentration. The properties of the toxin-resistant current are very similar to those of the currents expressed in oocytes following injection of alpha(1E) subunits which we demonstrated to be present in these neurons.Therefore a component of the toxin-resistant current calcium channels in sensory neurons may be closely related to those calcium channels formed by alpha(1E) subunits. (C) 1997 IBRO. Published by Elsevier Science Ltd.
